化疗对肿瘤患者淋巴细胞亚群的影响

目的探讨肿瘤患者化疗前后T淋巴细胞及亚群含量的变化及临床意义。方法采用流式细胞仪技术,分别对治疗组和对照组外周血T淋巴细胞及亚群进行检测计数。结果200例肿瘤患者CD3＋T淋巴细胞、CD4＋T淋巴细胞、NK细胞的数量以及CD4/CD8比值均明显低于健康人群。其中CD8＋T淋巴细胞的比例明显高于健康人群,差异具有统计学意义（P〈0.05）。有淋巴细胞转移组CD3、CD4、CD4/CD8及NK细胞数量较无淋巴细胞转移组明显下降,CD8显著升高,差异有统计学意义（P〈0.05）。化疗2个周期后。外周血CD3、CD4、NK细胞含量以及CD4/CD8比值化疗后较化疗前降低,差异均有统计学意义（P〈0.05）;化疗前后CD8均值变化不大（P〉0.05）。结论肿瘤患者机体的免疫状态与病情发展、临床分期密切相关,化疗可明显抑制肿瘤患者机体的免疫功能。

The Influence of Chemotherapy on the Lymphocyte Subsets in Patients with Tumor

Objective To evaluate the variations of lymphocyte and lymphocyte subsets before and after chemotherapy in patients with tumor.Methods Lymphocyte and lymphocyte subsets were determined by flow cytometry in 180 healthy controls and 200 patients with tumor before and after chemotherapy.Results Compared with the control group,the mean values of CD3,CD4,NK and CD4/CD8 ratio were significantly decreased,and the mean value of CD8 was significantly increased（P0.05） in patients with tumor.Patients with node metastases had significantly lower mean values of CD3,CD4,NK and CD4/CD8 ratio,and significantly higher mean CD8 values than those without node metastases（P0.05）.The mean value of CD3,CD4 and CD4/CD8 ratio were significantly decreased,but the mean CD8 values remained stable after chemotherapy（P0.05）.Conclusion The immunological status was correlated to the progression and clinical stage of tumor.Chemotherapy may inhibit the immunological response of patients with tumor.