子痫前期患者胎盘整合素α1与β1及肿瘤坏死因子α的表达及其临床意义

目的:探讨整合素α1、β1与肿瘤坏死因子α在子痫前期患者胎盘组织中的表达及其临床意义。方法:采用免疫组化SP法检测50例子痫前期患者(轻度20例,重度30例)胎盘组织中整合素α1、β1及肿瘤坏死因子α的表达;40例正常孕妇为对照组。结果:子痫前期组胎盘绒毛滋养细胞及蜕膜细胞整合素α1、β1的阳性表达率均明显低于对照组(整合素α1:58.00%和80.00%,P<0.05,整合素β1:56.00%和76.50%,P<0.05);肿瘤坏死因子α的阳性表达率明显高于对照组(肿瘤坏死因子α:80.00%和32.00%,P<0.05)。子痫前期组轻、重度患者整合素α1、β1及肿瘤坏死因子α表达的阳性率分别为整合素α1:75.00%和46.67%,整合素β1:75.00%和43.33%,并随病情程度加重而降低;肿瘤坏死因子α:65.00%和90.00%,并随病情程度加重而升高。整合素α1、β1的表达与胎盘重量及新生儿体重呈正相关。结论:胎盘表达整合素α1、β1与肿瘤坏死因子α异常引起滋养细胞浸润不足,可能是子痫前期的发病因素之一。

Expression and clinical significance of integrinα1, β1 and TNFα in placenta of preeclampsia

Objective:To study the expression and clinical significance of integrinα1,β1 and tumor necrosis factor(TNF)α in placentas of preeclampsia.Methods:Immunohistochemistry(SP) was used to determine the distribution and intensity of integrinα1,β1 and TNFα staining in placentas of 50 cases of preeclampsia patients(20 and 30 of mild and severe cases,respectively) and 20 cases of normal gravidas.Results:The expressions of integrinα1,β1 and TNFαin placental trophoblast and decidual cells were significantly decreased in preeclampsia group compared with those in normal group(integrinα1 : 58.00 % vs 80.00%,P < 0.05;integrinβ1:56.00% vs 76.50%,P<0.05;TNFα:80.00 % vs 32.00 %,P<0.05).In preeclampsia group,the expressions of integrinα1,β1,TNFα in mild and severe preeclampsia were : integrinα1 :75.00 % and 46.67 %;Integrinβ1: 75.00 % and 43.33%;TNFα: 65.00% and 90.00%.The expression of integrinα1,β1 was decreased with the progress of preeclampsia.Positive relationship was shown between the expression of integrinα1,β1 and the weight of placenta and neonate.Conclusion:The reduceed expression of integrinα1,β1 and the elevated expreesion of TNFαin placenta leads to insufficient trophoblasts infiltration and might be one of the pathogenesis of preeclampsia.