脑出血大鼠脑红蛋白表达与脑含水量变化

目的：观察脑出血大鼠脑组织内脑红蛋白（Ngb）的表达,探讨其与脑出血后脑水肿之间的关系。方法：将大鼠随机分为3组：正常对照组,假手术组和脑出血组。脑出血组和假手术组动物在模型建立后1、6、24、48、72h5个时间点各随机分为5个亚组。采用立体定向技术将自体血注入大鼠尾状核建立脑出血模型（假手术组注入生理盐水）,用干湿重法测定脑出血后脑含水量的变化,用Western blot检测脑出血后不同时间脑组织内Ngb的表达。结果：与假手术组或正常对照组比较,脑出血组1h时血肿周围脑组织内Ngb表达开始增高（P〈0.05）,24h时脑含水量开始升高（P〈0.05）,二者均在48h时达到高峰并持续至72h（P〈0.01）。结论：脑出血后脑组织内Ngb表达上调。脑出血后Ngb表达水平的变化与脑水肿的发生、发展在时间上不完全同步。

Relationship between expression of neuroglobin and changes of brain water content in rats with intracerebral hemorrhage

Objective To study the expression of neuroglobin (Ngb) in brain tissue in rats with intracerebral hemorrhage (ICH) and the relationship between the expression of Ngb and brain edema following ICH. Methods The rats were randomly divided into normal control group, sham-operated group and ICH group. Sham-operated group and ICH group were divided into 5 subgroups respectively according to 1, 6, 24, 48 and 72 h after the animal modal formation. ICH was induced by stereotaxic injection of autologous blood into right caudate nucleus of rats (saline was injected in sham-operated group). Wet and dry weight methods were used to measure the changes of brain water content, and Western blot were used to detect the expression of Ngb in brain tissue. Results Compared with normal control group or sham-operated group, the expression of Ngb in peri-hematomal brain tissue started to increase at 1 h (P<0.05), and the brain water content increased at 24 h in ICH group (P<0.05). Both peaked at 48 h and maintained the level until 72 h (P<0.01) in ICH group.Conclusion Expression of Ngb increases in brain tissue after ICH. The change of Ngb expression is not totally coincident with the formation of brain edema after ICH.