Loading of polystyrene solid phases with microbial agents and their polysaccharide antigens for the screening of monoclonal antibodies |
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Authors: | M Mehl R Starke A Urban A Hoffmann S Kiessig G Schmidt |
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Affiliation: | Institut für Medizinische Mikrobiologie, Humboldt-Universit?t zu Berlin. |
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Abstract: | Before screening monoclonal antibodies using enzyme immuno assays, it is necessary to prove that the coating of the solid phases occurs efficiently. The adhesion of whole cells of group B streptococci, Escherichia coli K1, Haemophilus influenzae type b and Candida albicans as well as B-Streptococcus group antigen, H. influenzae type b capsular antigen, and C. albicans cell wall antigen to polystyrene solid phases was studied and detected by the following methods: Peroxidase competition procedure, scintillation measurement of 32phosphate-labelled germs, and reactions with monoclonal antibodies. |
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