第二代杂交捕获法检测宫颈人乳头瘤病毒感染的初步研究

目的:探讨人乳头瘤病毒与宫颈癌及癌前期病变的相关性.方法:选取2004年12月～2005年7月我院妇科宫颈病门诊妇女1689例,取宫颈刷出物同时作宫颈薄层液基细胞学涂片与HPV-DNA检测,HPV-DNA检测方法是第二代杂交捕获法,可一次检测13种高危型HPV病毒,分析HPV感染与宫颈癌及癌前期病变的关系.结果:HPV-DNA检出率随宫颈癌变程度加重呈趋势性升高,1689例病人中高危型HPV检出率为25.16%(425/1689),宫颈癌及癌前期病变中为90.35%(234/259).结论:高危型HPV是诱发宫颈癌及癌前期病变的重要病因学因素.第二代杂交捕获法可作为宫颈癌的一种筛查方法.

Preliminary Study of Cervical Human Papillomavirus Infection Using Hybrid Capture Ⅱ

Objective: To investigate the relationship between human papillomavirus and cervical carcinoma and cervical intraepithelial neo-plasia. Methods:From Decemder 2004 to July 2005,1689 outpatient women were screened high risk types of human papillomavirus(HPV) u-sing Hybrid Capture II and cervical cytology using the ThinPrep Pap Smear fluid-based system. The Hybrid Capture E can detect 13 types of high-risk HPV DNA. Meanwhile, colposcopy and histological examination on cervical tissue were performed on all patients. Results: HPV infection rates increased according to the seriousness degree of cervical lesions. The infection rate of high-risk HPV DNA of all patient was 25. 16 %(425/1689),the patient of cervical carcinoma and cervical intraepithelial neoplasia was 90. 35% (234/259). Conclusion; High-risk HPV is the major cause of cervical carcinoma and cervical intraepithelial neoplasia. So the Hybrid Capture II could be used for screening primary cervical carcinoma.