| 食管癌Eca109细胞中Nucleostemin基因表达的实验研究 |
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| 引用本文： | 郑学芝,金秀东,张绪东,刘桂莲,孙卫,念红,李丽,徐秋玲,蔡子微.食管癌Eca109细胞中Nucleostemin基因表达的实验研究[J].牡丹江医学院学报,2011,32(1):3-5. |
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| 作者姓名： | 郑学芝 金秀东 张绪东 刘桂莲 孙卫 念红 李丽 徐秋玲 蔡子微 |
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| 作者单位： | 1. 牡丹江医学院生理教研室,黑龙江,牡丹江,157011;黑龙江省高校肿瘤疾病防治重点实验室,黑龙江,牡丹江,157011
2. 黑龙江省高校肿瘤疾病防治重点实验室,黑龙江,牡丹江,157011 |
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| 基金项目： | 黑龙江省普通高等学校青年学术骨干支持计划项目 |
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| 摘 要： | 目的：研究Nucleostemin（NS）基因在食管癌Eca109细胞中的表达及凋亡作用。方法：提取各组食管癌细胞总RNA,用半定量RT-PCR方法检测NS基因的表达情况;用CCK-8法检测3组细胞增殖抑制率;TUNEL试剂盒法检测细胞凋亡情况。结果：与对照组比较,实验组细胞NS基因表达量下降,细胞增殖抑制率〉65%,细胞凋亡增加（凋亡指数为39.47%）差别具有统计学意义。结论：NS基因特异性RNA干扰使食管癌Eca109细胞中NS基因表达量下降,出现细胞增殖抑制,细胞凋亡增加。
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| 关 键 词： | Nucleostemin 食管癌 RNA干扰 凋亡 |
THE EXPERIMENTAL STUDY OF NUCLEOSTEMIN GENE EXPRESSION IN ESOPHAGEAL CANCER ECA109 CELLS |
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| Zheng Xue-zhi et al..THE EXPERIMENTAL STUDY OF NUCLEOSTEMIN GENE EXPRESSION IN ESOPHAGEAL CANCER ECA109 CELLS[J].Journal of Mudanjiang Medical College,2011,32(1):3-5. |
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| Authors: | Zheng Xue-zhi |
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| Institution: | Zheng Xue-zhi et al.(Department of Physiology,Mudanjiang Medical college,Mudanjiang 157011 china) |
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| Abstract: | Objective:To detect the NS gene expression and cell apoptosis in esophageal cancer Eca109 cells.Methods: Total RNA was isolated from esophageal cancer Eca109 cells,measured the NS gene expression by RT-PCR;then detected the cell proliferation by CCK-8 assay;detected the cell apoptosis by TUNEL kit.Results:compared with the two control groups,the NS gene expression in silencer group cells reduced,the proliferation inhibitory rate was higher than 65%,cell apoptosis increased.Conclusion:the NS gene specific RN... |
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| Keywords: | Nucleostemin |
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