| HPLC法测定化瘀无糖颗粒中人参皂苷Rg_1、Re含量 |
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| 引用本文： | 陈金娜,刘向红.HPLC法测定化瘀无糖颗粒中人参皂苷Rg_1、Re含量[J].山东中医药大学学报,2013(3):236-238. |
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| 作者姓名： | 陈金娜 刘向红 |
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| 作者单位： | 山东中医药大学;山东大学齐鲁医院 |
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| 基金项目： | 山东省自然科学基金资助项目(编号:Y2006C62) |
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| 摘 要： | 目的:建立化瘀无糖颗粒中人参皂苷Rg1、Re含量的HPLC测定方法。方法 :色谱柱:Phenomenex Luna C18(4.6×250 mm,5μm);流动相:乙腈-0.1%磷酸水梯度洗脱;流速:1.0 mL/min;检测波长:203 nm;柱温:25℃。结果:人参皂苷Rg1线性范围为0.326～5.23μg(r=0.999 9),平均回收率99.38%(RSD 0.98%,n=6);人参皂苷Re线性范围为0.118～1.904μg(r=0.999 8),平均回收率99.08%(RSD 1.66%,n=6)。结论:样品处理方法合理,方法学考察符合定量要求,结果准确,可用于化瘀无糖颗粒中人参皂苷Rg1、Re的含量测定。
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| 关 键 词： | 化瘀无糖颗粒 人参皂苷Rg1、Re HPLC |
Determination of ginsenosides Rg_1,Re of Huayu Wutang granules by HPLC |
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| Institution: | CHEN Jin- na1,LIU Xiang- hong2(1.2010 Master Degree Candidate of Shandong University of Traditional Chinese Medicine,Jinan 250355,China;2.Qilu Hospital of Shandong University,Jinan 250012,China) |
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| Abstract: | Objective:To establish the determination methods of the content of ginsenosides Rg1,Re of Huayu Wutang granules by HPLC.Methods:Phenomenex Luna C18 column(4.6 mm×250 mm,5 μm),mobile phase acetonitrile-0.1% phosphoric acid solution gradient elution,flow rate 1.0 mL/min the detection wavelength 203 nm,column temperature 25 ℃.Results:Linear relationship of ginsenoside Rg1 in the range of 0.326～5.23 μg(r=0.9999) was good,average recovery was 99.38%(RSD 0.98%,n=6),linear relationship of ginsenoside Re in the range of 0.118～1.904 μg(r=0.9998) was good,average recovery was 99.08%(RSD 1.66%,n=6).Conclusion:Sample handing method was reasonable,methodological study meeting the quantitative requirements,accurate results can be used in Huayu Wutang granules in the content determination of ginsenosides Rg1,Re. |
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| Keywords: | Huayu Wutang granules ginsenosides Rg1 and Re HPLC |
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