| 环氧化酶抑制剂对人乳腺癌细胞株MCF-7的抑制作用研究 |
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| 引用本文： | 刘方欣,张玉诺,李少林. 环氧化酶抑制剂对人乳腺癌细胞株MCF-7的抑制作用研究[J]. 重庆医科大学学报, 2005, 30(4): 564-566,580 |
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| 作者姓名： | 刘方欣 张玉诺 李少林 |
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| 作者单位： | 重庆医科大学基础医学院核医学教研室,重庆,400016 |
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| 摘 要： | 目的:研究环氧化酶抑制剂阿司匹林和选择性环氧化酶2抑制剂尼美舒利对人乳腺癌细胞株MDA-MB-231的生长抑制作用,并初步探讨其作用机理.方法:用阿司匹林和尼美舒利分别作用人乳腺癌细胞株MDA-MB-231,MTT法观察其生长抑制作用;免疫组化法检测阿司匹林和尼美舒利作用后MDA-MB-231细胞中COX-2和c-erbB-2的表达情况.结果:阿司匹林和尼美舒利能够抑制MDA-MB-231细胞的生长,并呈时间-剂量依赖型.COX-2在MDA-MB-231细胞中表达呈阳性,免疫组化发现细胞内c-erbB-2表达下降.结论:环氧化酶抑制剂能够有效的抑制肿瘤细胞的生长,其机制可能与下调c-erbB-2基因的表达有关.
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| 关 键 词： | 环氧化酶抑制剂 选择性环氧化酶2抑制剂 人乳腺癌细胞株MDA-MB-231 |
| 文章编号： | 0253-3626（2005）04-0564-03 |
| 收稿时间： | 2004-09-13 |
| 修稿时间： | 2004-09-13 |
The inhibitory effect of cyclooxygenase inhibitor on human breast cancer line MDA-MB- 231 in vitro |
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| Liu FangXin;Zhang YuNuo;Li ShaoLin. The inhibitory effect of cyclooxygenase inhibitor on human breast cancer line MDA-MB- 231 in vitro[J]. Journal of Chongqing Medical University, 2005, 30(4): 564-566,580 |
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| Authors: | Liu FangXin Zhang YuNuo Li ShaoLin |
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| Abstract: | Objective:To investigate the inhibitory effect of cyclooxygenase inhibitor acetylsalicylic acid(ASA),and a selective COX-2 inhibitor(Nimesulide) on the growth of human breast cancer cell line MDA-MB-231 in vitro.Methods:The inhibitory effect was detected by MTT assay.Immunohistochemical assay was performed to examine the expressions of COX-2 and c-erbB-2 in SKOV3 cell line treated by NSAIDs.Results:A decrease in cell number compared with controls was observed in all of the cell line treated with ASA and Nimesulide.It was a dose-dependent inhibition of cell proliferation.COX-2 was expressed positive in MDA-MB-231 and the expression of cerbB-2 was found to decrease by immunohistochemical assay.Conclusion:NSAIDs can inhibit the growth of human breast cancer cell line MDA-MB-231 in vitro. |
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| Keywords: | Cyclooxygena inhibitor acetylsalicylic acid Selective COX- 2 inhibitor Human breast cancer cell line MDA- MB-231 |
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