中华眼镜蛇毒对荷人鼻咽癌裸鼠血过氧化氢酶和GSH-PX活性的影响

目的:探讨中华眼镜蛇毒(NNAV)对荷人鼻咽癌(NPC)裸鼠血中过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-PX)活性的影响。方法:采用比色法检测经腹腔注射低、中或高浓度NNAV(1mg／L、5mg／L或10mg／L)的荷人NPC裸鼠血中CAT和GSH-PX活性。结果:荷人NPC裸鼠组血中CAT活性(16450U/L)低于正常裸鼠组(20680U/L)(P＜0.05),而荷瘤裸鼠组血中GSH-PX活性(27670U/L)与正常裸鼠组(28790U/L)相比P＞0.05。低、中和高浓度NNAV治疗后的荷瘤鼠组血中CAT活性(20570U/L、23090U/L、21280U/L)均高于荷瘤鼠组(16450U/L)(P＜0.05),并接近正常水平(20680U/L)(P＞0.05)。同时低、中和高浓度用药组血中GSH-PX活性均不同程度地高于荷瘤鼠组(27670U/L)(P＜0.05),其中高浓度组(37810U/L)明显高于低或中浓度组(29940U/L、30610U/L)(P＜0.05),其血中GSH-PX活性明显高于正常裸鼠组(28790U/L)(P＜0.05)。结论:NNAV的抑瘤作用可能与其提高荷瘤鼠血中CAT和GSH-PX活性有关。

Effect of naja naja atra venom on plasmic GSH-PX and catalase of human

AIM: To investigate the changes of plasma glutathione peroxidase (GSH-PX) and catalase (CAT) activities in nude mice (NM) bearing human nasopharyngeal carcinoma (NPC) and observe the effect of naja naja atra venom (NNAV) on them. METHODS: Plasma GSH-PX and CAT activities in human NPC bearing NM treated ( i.p. ) by low, middle or high concentration NNAV solution (1 mg/L, 5 mg/L, 10 mg/L) were determined by colorimetry. RESULTS: Plasma CAT activity (16 450 U/L) in NM bearing tumor group decreased significantly in comparison with the control group (20 680 U/L)(P<0.05). Plasma GSH-PX activity (27 670 U/L) in NM bearing tumor group had no apparent alteration in comparison with the control group (28 790 U/L)(P>0.05). Treated by low, middle or high concentration NNAV solution, CAT activities of three NM bearing tumor groups (20 570 U/L, 23 090 U/L, 21 280 U/L ) were higher than that of the NM bearing tumor group without NNAV treatment (16 450 U/L) (P<0.05), and reached to control level (20 680 U/L)(P>0.05). GSH-PX activities of the three groups (especially high concentration group) were higher than that of the group without NNAV treatment (P<0.05). CONCLUSION: The antitumor effect of NNAV might be by means of increasing GSH-PX activity and CAT activity in NM bearing human NPC.