| 心外膜细胞标记基因介导的Cre重组酶在双转基因小鼠心脏的表达 |
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| 引用本文： | 曾彬,武智晓,张静,易欣,马乐乐. 心外膜细胞标记基因介导的Cre重组酶在双转基因小鼠心脏的表达[J]. 微循环学杂志, 2011, 21(3): 12-13,88,90,85 |
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| 作者姓名： | 曾彬 武智晓 张静 易欣 马乐乐 |
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| 作者单位： | 武汉大学人民医院心血管内科,武汉,430060 |
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| 基金项目： | 国家自然科学基金青年基金 |
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| 摘 要： | 目的:检测心外膜细胞标记基因Tbx18介导的Cre重组酶在双转基因小鼠心脏的表达及分布。方法:将Tbx18-Cre小鼠与ROSA26报告小鼠交配,收获不同怀孕天数(12.5天、13.5天、14.5天)的胚胎,用PCR技术筛选双转基因阳性胚胎,取阳性胚胎心脏进行冰冻切片、X-Gal染色。结果:PCR检测每只小鼠胚胎中约一半胚胎为双转基因阳性,这些胚胎心脏的心外膜、左心室壁及室间隔均可检测到X-Gal阳性的蓝色细胞;且随着胚胎心脏的发育,蓝色细胞显著增多。结论:心外膜细胞标记基因Tbx18介导的Cre重组酶能有效地在双转基因胚胎小鼠心脏表达,表明Tbx18在心脏发育过程中扮演重要角色。
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| 关 键 词： | 心外膜细胞 标记基因Tbx18 Cre重组酶 双转基因小鼠 |
Expression and Distribution of the Cre Recombinase Activity in Transgenic Mouse Line Under the Control of Epicardial Cell Marker |
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| Zeng Bin,Wu Zhixiao,Zhang Jing,et al/. Expression and Distribution of the Cre Recombinase Activity in Transgenic Mouse Line Under the Control of Epicardial Cell Marker[J]. Chinese Journal of Microcirculation, 2011, 21(3): 12-13,88,90,85 |
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| Authors: | Zeng Bin Wu Zhixiao Zhang Jing et al/ |
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| Affiliation: | Zeng Bin,Wu Zhixiao,Zhang Jing,et al/Department of Cardiology,Renmin Hospital of Wuhan University,Wuhan 430060 |
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| Abstract: | Objective: To identify the expression and distribution of the Cre recombinase activity in transgenic mouse line(Tbx18-Cre) under the control of epicardial cell marker Tbx18.Method: Tbx18-Cre transgenic mice were bred with ROSA26 reporter strain,and mouse embryonic hearts were collected at different stages for OCT embeding.At the same time,the tail tissues of the embyonic heart were used to identify the gene type by PCR.The positive one was used to the staining of X-Gal.Results: PCR results revealed that a h... |
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| Keywords: | Epicardial cells Marker gene Tbx18 Cre recombinase Double transgenic mice |
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