| hVEGF165在自体骨髓间充质干细胞移植治疗兔缺血后肢中的表达 |
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| 引用本文： | 杜金刊,王宾,沈来根. hVEGF165在自体骨髓间充质干细胞移植治疗兔缺血后肢中的表达[J]. 医学研究杂志, 2008, 37(6): 39-41 |
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| 作者姓名： | 杜金刊 王宾 沈来根 |
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| 作者单位： | 1. 浙江省东阳市人民医院,322100
2. 浙江大学医学院附属邵逸夫医院,杭州,310016 |
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| 基金项目： | 浙江省金华市科学技术计划研究项目 |
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| 摘 要： | 目的 观察hVEGF165在自体骨髓间充质干细胞移植治疗兔缺血后肢中的表达.方法 体外培养兔骨髓问充质干细胞,通过腺病毒载体转染hVEGF165基因.移植后4周内,采用RT-PCR法鉴定hVEGF165在兔缺血后肢中的表达.结果 hVEG165成功转染骨髓间充质干细胞,移植后在兔缺血后肢中有效表达.结论 hVEGF165转染干细胞后能在体内有效表达.
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| 关 键 词： | 骨髓间充质干细胞 血管内皮生长因子 表达 自体骨髓 骨髓间充质干细胞 细胞移植治疗 缺血后 有效表达 Mesenchymal Stem Cells Transplantation Model Rabbit Limb Bind Endothelial Growth Factor Vascular Human 结果 基因 转染 毒载体 骨髓问充质干细胞 养兔 |
Expression of Human Vascular Endothelial Growth Factor 165 in Ischemically Damaged Bind Limb in a Rabbit Model with Transplantation of Mesenchymal Stem Cells |
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| Du Jinkan,Wang Bin,Shen Laigen. Expression of Human Vascular Endothelial Growth Factor 165 in Ischemically Damaged Bind Limb in a Rabbit Model with Transplantation of Mesenchymal Stem Cells[J]. Journal of Medical Research, 2008, 37(6): 39-41 |
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| Authors: | Du Jinkan Wang Bin Shen Laigen |
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| Abstract: | Objective To observe the expression of Vascular endothelial growth factor 165 (VEGF165) in transplantation of MSCs to repair an ischemically damaged bind limb in a rabbit model.Methods MSCs were cultivated in vitro and transfected with non-replicative adenoviral vector with VEGF165 gene. Four weeks after treatment, Real-time PCR assessed the expression of VEGF165 in the damaged limb.Results It was confirmed that VEGF165 was successfully transfected into MSCs.Real-time PCR assessments revealed the expression... |
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| Keywords: | Mesenchymal stem cells VEGF165 Expression |
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