促红细胞生成素对新生HIBD大鼠凋亡相关蛋白Bax表达的影响

目的观察重组人促红细胞生成素(rhEPO)对新生大鼠缺氧缺血性脑损伤(HIBD)后凋亡相关蛋白Bax表达的影响,探讨rhEPO发挥神经保护作用的可能机制。方法将新生7日龄SD大鼠120只随机分为3组,假手术组、对照组、rhEPO治疗组。其中rhEPO治疗组,在建立新生大鼠HIBD模型后,即刻一次性给予rhEPO 3000U/kg颈部皮下注射,用HE及免疫组化方法观察给药后不同时间点凋亡相关蛋白Bax表达的变化。结果新生7日龄大鼠HIBD后神经细胞凋亡增加,凋亡蛋白Bax的表达在各时间点较rhEPO治疗组和假手术增加(P<0.01),rhEPO干预后凋亡相关蛋白Bax表达较对照组明显减少(P<0.01),差异有统计学意义。结论 rhEPO可以通过下调凋亡相关蛋白Bax的表达,抑制细胞凋亡而发挥其神经保护作用,为临床治疗新生儿缺氧缺血性脑病(HIE)提供有意义的理论依据。

Effect of erythropoietin on expression of apoptosis associated protein in neonatal rats with HIBD

Objective To study the effect and the molecular mechanisms of erythropoietin （EPO） on the expression of apoptosis associated ischemic brain damage. Methods Seven-day-old pr SD otein Bax in neonatal rats following hypoxic- rats were randomly divided into three group： sham-operated group,control group and rhEPO-treated group. In the rhEPO-treated group, in stantly give single injection rhEPO 3000U/kg, measuring the expression of apoptosis associated protein Bax at different time afer injection by HE and immunohistochemical method. Results Compare with rhEPO-treated group and sham ated pro day-old operated group, the expression of apoptosis associ tein Bax and the nerve cell apoptosis were increased afer HIBD at different time in seven Wistar rats（P〈0.01）. To compare with control group, the expression of apoptosis asso ciated protein Bax in rhEPO-treated group was significantly lower, the difference had statistical significance. Conclusion The neuroprotective effect of rhEPO may be releated to down regula- tion the expression of apoptosis associated protein Bax, provided the significant theory for clinical treatment of newborn hypoxie-ischemie encephalopathy.