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蒲黄的黄酮类成分提取工艺研究
引用本文:傅大煦,王玮,程小卫,陈家宽,周铜水. 蒲黄的黄酮类成分提取工艺研究[J]. 中成药, 2005, 27(5): 517-520
作者姓名:傅大煦  王玮  程小卫  陈家宽  周铜水
作者单位:1. 上海新药研究开发中心,上海,201203
2. 复旦大学生命科学学院,上海,200433
摘    要:目的:优选蒲黄总黄酮的提取工艺条件.方法:以异鼠李素-3-0-新陈皮糖苷和香蒲新苷含量为指标,应用L27(313)正交试验优化蒲黄总黄酮的提取方法.结果:乙醇浓度、提取时间、提取次数对提取效率有显著性影响.结论:最佳提取工艺为用14倍生药量的40%乙醇提取两次,每次为3 h.

关 键 词:蒲黄  总黄酮  正交试验  提取工艺
文章编号:1001-1528(2005)05-0517-03
修稿时间:2004-03-25

Extraction process of the total flavonoids of Pollen typhae
FU Da-xu,WANG Wei,CHENG Xiao-Wei,CHEN Jia-Kuan,ZHOU Tong-shui. Extraction process of the total flavonoids of Pollen typhae[J]. Chinese Traditional Patent Medicine, 2005, 27(5): 517-520
Authors:FU Da-xu  WANG Wei  CHENG Xiao-Wei  CHEN Jia-Kuan  ZHOU Tong-shui
Abstract:AIM: To optimize the extraction process of the total flavonoids of Pollen typhae. METHODS: The optimum extraction process was selected with the orthogonal design (L 27 (3 13 )), using the contents of typhaneoside and isorhamnetin 3 O neohesperidoside as the evaluating criteria. RESULTS: The significant effects of alcohol concentration, extraction time and extraction times on the extraction of the total flavonoids of Pollen typhae were discovered. CONCLUSION: The optimum extraction process was as follows: Pollen typhae was extracted with 40% alcohol for two times, each with the solvent volume 14 times of the weight of the raw materials and extraction time for 3 h.
Keywords:Pollen typhae  total flavonoids  orthogonal design  extraction
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