| Establishment of cervical lymph node metastasis model of squamous cell carcinoma in the oral cavity in mice |
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| 作者单位： | SUN Rui,GUO Chuan-bin(Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University, Beijing 100081,China);ZHANG Jian-gang(Department of Pathology, School of Basic Medical Sciences,Lanzhou University, Lanzhou, Gansu 730000, China) |
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| 基金项目： | Capital Development Fund, Beijing,No. 2003-3009; |
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| 摘 要： | Background Oral squamous cell carcinoma （OSCC） is the most prevalent malignant tumor in the head and neck region, comprising more than 90% of all oral malignancies. A feasible approach for an animal model to study OSCC lymph node metastasis was established and biological behaviors of three oral squamous cell carcinoma cell lines were compared. Methods After implanting three kinds of cell lines （GDC185, Tca8113, Tca83） into three different anatomical sites in nude mice, namely the tongue, floor of the mouth, and axillary fossa, we observed the tumorigenicity and the metastatic capacity, which was confirmed by histopathology under a surgical microscope. Results The animal model injected with GDC185 cells into the floor of the mouth had the highest rate of neck lymph node metastasis （55.6%） and the cell lines had significantly different biological behaviors. Conclusions Nude mice injected with GDC185 cells into the floor of the mouth could be used as a feasible animal model to study neck metastasis of oral squamous cell carcinoma.
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| 关 键 词： | 口腔肿瘤 鳞状细胞癌 淋巴节转移 动物模型 |
Establishment of cervical lymph node metastasis model of squamous cell carcinoma in the oral cavity in mice |
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| Authors: | SUN Rui ZHANG Jian-gang GUO Chuan-bin |
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| Institution: | 1. Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University, Beijing 100081,China
2. Department of Pathology, School of Basic Medical Sciences,Lanzhou University, Lanzhou, Gansu 730000, China |
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| Abstract: | Background Oral squamous cell carcinoma (OSCC) is the most prevalent malignant tumor in the head and neck region, comprising more than 90% of all oral malignancies. A feasible approach for an animal model to study OSCC lymph node metastasis was established and biological behaviors of three oral squamous cell carcinoma cell lines were compared. Methods After implanting three kinds of cell lines (GDC185, Tca8113, Tca83) into three different anatomical sites in nude mice, namely the tongue, floor of the mouth, and axillary fossa, we observed the tumorigenicity and the metastatic capacity, which was confirmed by histopathology under a surgical microscope.Results The animal model injected with GDC185 cells into the floor of the mouth had the highest rate of neck lymph node metastasis (55.6%) and the cell lines had significantly different biological behaviors. Conclusions Nude mice injected with GDC185 cells into the floor of the mouth could be used as a feasible animal model to study neck metastasis of oral squamous cell carcinoma. |
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| Keywords: | animal model lymphatic metastasis squamous cell carcinoma mouth neoplasm |
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