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| cAMP反应元件结合蛋白在高糖所致内皮细胞损伤中的作用 | |
| 引用本文： | 屈顺林,范文静,张弛,郭芳,罗其富,姜志胜.cAMP反应元件结合蛋白在高糖所致内皮细胞损伤中的作用[J].中国动脉硬化杂志,2012,20(6):494-498. |
| 作者姓名： | 屈顺林 范文静 张弛 郭芳 罗其富 姜志胜 |
| 作者单位： | 1. 南华大学基础医学博士后流动站,湖南省衡阳市421001;南华大学病理生理学教研室心血管疾病研究所动脉硬化学湖南省重点实验室,湖南省衡阳市421001 2. 南华大学附属第二医院,湖南省衡阳市,421001 3. 南华大学病理生理学教研室心血管疾病研究所动脉硬化学湖南省重点实验室,湖南省衡阳市,421001 |
| 基金项目： | 国家自然基金项目，中国博士点基金新教师类联合项目，中国博士后科学基金项目，湖南省教育厅科研项目，南华大学高层次人才科研启动项目 |
| 摘 要： | 目的探讨cAMP反应元件结合蛋白(CREB)在高糖致内皮细胞损伤中的作用。方法建立高糖诱导的血管内皮细胞损伤模型,将内皮细胞ECV304分为对照组、高糖组、转染PCI组、转染PCI+高糖组、转染PCI-CREB组和转染PCI-CREB+高糖组,采用MTT法检测细胞存活率,流式细胞仪检测细胞凋亡率,硫代巴比妥酸法检测丙二醛(MDA)含量,黄嘌呤氧化酶法检测细胞超氧化物歧化酶(SOD)活性。结果转染PCI-CREB组和转染PCI组内皮细胞存活率、SOD活性、MDA含量和细胞凋亡率与对照组相比差异无统计学意义(P>0.05);与对照组相比,高糖组内皮细胞存活率和SOD活性明显下降,而MDA含量和细胞凋亡率明显增加(P<0.05);转染PCI+高糖组内皮细胞存活率、SOD活性、MDA含量和细胞凋亡率与高糖组相比差异无统计学意义(P>0.05);与高糖组相比,转染PCI-CREB+高糖组细胞存活率和SOD活性明显高于高糖组(P<0.05),而MDA含量和细胞凋亡率明显低于高糖组(P<0.05)。结论过表达CREB对高糖诱导的内皮细胞损伤具有保护作用。 |
| 关 键 词： | cAMP反应元件结合蛋白 高糖 内皮细胞 细胞凋亡 |
| 收稿时间： | 2011/11/6 0:00:00 |
Role of cAMP Response Element Binding Protein in Endothelial Cells Injury Induced by High Glucose |
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| QU Shun-Lin,FAN Wen-Jing,ZHANG Chi,GUO Fang,LUO Qi-Fu,and JIANG Zhi-Sheng.Role of cAMP Response Element Binding Protein in Endothelial Cells Injury Induced by High Glucose[J].Chinese Journal of Arteriosclerosis,2012,20(6):494-498. | |
| Authors: | QU Shun-Lin FAN Wen-Jing ZHANG Chi GUO Fang LUO Qi-Fu and JIANG Zhi-Sheng |
| Institution: | 1,2(1.Post-Doctoral Mobile Stations for Basic Medicine,2.Department of Pathophysiology,Institute of Cardiovascular Disease & Key Laboratory for Arteriosclerology of Hunan Province,3.The Second Affiliated Hospital,University of South China,Hengyang,Hunan 421001,China) |
| Abstract: | Aim To investigate the role of cAMP response element binding protein(CREB) in endothelial cells injury induced by high glucose.Methods The endothelial cell injury model was induced by high glucose.ECV304 cells were divided into six groups: control group,high glucose group,PCI transfection group,PCI transfection+high glucose group,PCI-CREB transfection group and PCI-CREB transfection+high glucose group.The cell survival rate of ECV304 cells was detected by MTT method.Flow cytometry was used to detect the apoptosis rate.The MDA concentration was observed by TBA method and SOD activity was tested by xanthine oxidase method.Results Compared with control group,cell survival rate,SOD activity,MDA concentration and apoptosis rate in PCI-CREB transfection group and PCI transfection group were not significantly different(P>0.05).Compared with control group,both cell survival rate and SOD activity in high glucose group showed a significant decrease,however,apoptosis rate and MDA concentration increased significantly(P<0.05).Cell survival rate,SOD activity,MDA concentration and apoptosis rate in PCI transfection+high glucose group was not significantly different compared with high glucose group(P<0.05).Compared with high glucose group,both cell survival rate and SOD activity in PCI-CREB transfection+high glucose group showed a significant increase,however,apoptosis rate and MDA concentration decreased significantly(P<0.05).ConclusionOverexpression of CREB may protect endothelial cells from high glucose injury. |
| Keywords: | cAMP Response Element Binding Protein High Glucose Endothelial Cell Cell Apoptosis |
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