高表达整合素连接激酶促进脐静脉内皮细胞增殖

目的探讨高表达整合素连接激酶对体外培养人脐静脉内皮细胞增殖的影响。方法利用高表达整合素连接激酶的腺病毒转染脐静脉内皮细胞,诱导其在脐静脉内皮细胞中高表达。采用噻唑蓝法、流式细胞术、EDU法检测脐静脉内皮细胞增殖能力;Western blot检测脐静脉内皮细胞蛋白激酶B(Akt)、内皮型一氧化氮合酶(eNOS)蛋白表达及其蛋白磷酸化水平。结果整合素连接激酶在脐静脉内皮细胞高表达后,噻唑蓝法、流式细胞术、EDU法检测结果均表明:整合素连接激酶病毒转染组细胞增殖能力明显高于空病毒转染对照组(P<0.05或P<0.01)。Western blot检测发现整合素连接激酶病毒转染组蛋白激酶B、内皮型一氧化氮合酶磷酸化水平较对照组明显升高(P<0.05),而蛋白激酶B、内皮型一氧化氮合酶的表达水平两组间无差异。结论整合素连接激酶能促进脐静脉内皮细胞增殖,其机制可能通过激活下游Akt/eNOS通路。

Overexpression Integrin-linked Kinase Promotes Human Umbilical Vein Endothelial Cells Proliferation

Aim To investigate the proliferation effects of overexpression integrin-linked kinase on human umbilical vein endotheial cells(HUVEC).Methods HUVEC were infected by integrin-linked kinase(ILK) Ad-virus and induced ILK overexpression.HUVEC proliferation levels were measured by thiazolyl blue(MTT),flow cytometry and 5-ethynyl-2’-deoxyuridine(EDU) assay.Western blot was used to assess protein kinase B(Akt),endothelial nitric oxide synthase(eNOS) expression and phosphorylation levels.Results MTT assay,flow cytometry cycle,EDU assay all showed that cells proliferation were significantly higher in ILK overexpressed group than in the control group(P<0.01 or P<0.05).The levels of Akt and eNOS phosphorylation levels were also significantly higher than that in control group(P<0.05),but Akt and eNOS expression levels had no difference between the two groups.Conclusions ILK overexpression can promote HUVECs proliferation.This effect may be mediated through actvation of Akt and eNOS.