miR-19a在鼻咽癌细胞上皮间质转化中的作用

目的 研究微小RNA-19a(miR-19a)在鼻咽癌细胞中的表达以及对体外鼻咽癌细胞上皮间质转化(EMT)的作用.方法 利用实时荧光定量PCR(RT-qPCR)法检测miR-19a在鼻咽癌细胞株CNE2、SUNE-1、HNE1、58F及人正常鼻咽上皮细胞株NP69中的转录水平;使用miR-19a模拟物(miR-19a...

Role of miR-19a in the epithelial-to-mesenchymal-transition (EMT) of nasopharyngeal cancer

ABSTRACT] AIM: To investigate the expression of miR-19a in four nasopharyngeal cancer cell lines and the function of miR-19a on the process of nasopharyngeal cancer EMT. METHODS: The relative level of miR-19a in a panel of nasopharyngeal cancer cell lines was assessed by RT-qPCR, namely CNE2, SUNE-1, HNE1 and 58F, along with NP69, which is a normal human nasopharyngeal epithelial cell line. MiR-19a-overexpression SUNE-1 cells and miR-19a-knockdown HNE1 cells were established by transiently transfection with miR-19a mimics and miR-19a inhibitor, respectively. The migratory capacity of nasopharyngeal cancer cells was assessed using transwell migration chambers. Western blot was employed to analyze the effect of miR-19a on the EMT process of nasopharyngeal cancer cells. Bioinformatics software TargetScan was employed to detect the possible target gene of miR-19a, and such predication was verified by luciferase reporter experiment. Following, the rescue experiments were used to confirm whether miR-19a regulates EMT of nasopharyngeal cancer by affecting the target gene directly. RESULTS: The level of miR-19a in NP69 cells was almost 5.88, 3.57, 11.11 and 6.67 times than that in CNE2, SUNE-1, HNE1 and 58F cells, respectively. After transfection with miR-19a mimic, the migratory capacity of SUNE-1 cells decreased significantly (25.6±4.4) vs (10.5±2.1), P < 0.01]. On the contrary, down-regulation of miR-19a dramatically promoted the migratory capacity of HNE1 cells (16.9±2.5) vs (34.2±4.9), P < 0.01]. Biology information forecast the target genes and luciferase assay confirmed that KRAS was a direct target gene of miR-19a. The invaded cells in mimics NC, miR-19a mimics, miR-19a mimics+KRAS groups were (28.3±3.4), (11.6±1.5) and (23.7±2.8), respectively. The promotion of KRAS could reverse the inhibitory effect of miR-19a on the invasion of nasopharyngeal cancer cells. Western blot experiment confirm that the levels of N-cadherin, p-FAK and p-SRC were inhibited after upregulation of miR-19a, whereas the expression of E-cadherin was increased. The regulatory effect of miR-19a on the levels of E-cadherin, N-cadherin, p-FAK and p-SRC was attenuated by the promotion of KRAS. CONCLUSION: MiR-19a inhibits the EMT process of nasopharyngeal cancer cells. The tumor suppressor function, at least partly, is realized by targeting gene KRAS and negatively regulate the FAK/SRC signaling pathway.