| 精子冻融技术及其研究进展 |
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| 引用本文： | 陈洋,刘睿智. 精子冻融技术及其研究进展[J]. 中华男科学杂志, 2007, 13(8): 734-738 |
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| 作者姓名： | 陈洋 刘睿智 |
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| 作者单位： | 吉林大学基础医学院细胞生物学教研室,吉林省生殖医学研究所,吉林,长春,130021 |
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| 摘 要： | 精子冻融是辅助生殖技术的基础,冻融可能造成精子结构损伤和活力功能丧失,因此优化冻融方法十分必要。提高冷冻前精液质量、进行精子优选、添加合适的冷冻保护剂、选择适宜的冷冻复苏方法等均可有效地减少冻融损伤,有利于保存男性生育力。本文对精子冻融的基本原理、方法改良、针对特殊质量精液的冷冻方法及冷冻后精子质量评估进行了综述。
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| 关 键 词： | 精子冻融 优选技术 保护剂 冷冻损伤 冷冻评估 |
| 文章编号： | 1009-3591（2007）08-0734-05 |
| 收稿时间： | 2006-09-20 |
| 修稿时间： | 2006-09-202007-01-30 |
Cryopreservation of Spermatozoa |
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| CHEN Yang,LIU Rui-zhi. Cryopreservation of Spermatozoa[J]. National journal of andrology, 2007, 13(8): 734-738 |
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| Authors: | CHEN Yang LIU Rui-zhi |
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| Affiliation: | Department of Cell Biology, College of Basic Medicine, Jilin University, Changchun, Jilin 130021, China |
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| Abstract: | Sperm cryopreservation has revolutionized the field of assisted reproduction. However, it causes cryodamage to the structure and function of spermatozoa during the freezing-thawing process. Optimization of sperm cryopreservation is necessary for the preservation of male fertility. Cryodamage can be reduced effectively by such methods as improvement of semen quality before freezing, spermatozoal selection, addition of optimal cryoprotectants and application of appropriate thawing techniques. Recent studies focus on cryobiology, improvement of freezing-thawing methods, especially for poor quality semen, and evaluation criteria for post-thaw spermatozoa. |
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| Keywords: | sperm cryopreservation selected method cryoprotectant cryodamage freezing-thawing evaluation |
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