RP—HPLC法测定人血浆中盐酸二甲双胍的血药浓度

目的建立人血浆样品中盐酸二甲双胍浓度的含量测定方法。方法血浆以10％三氯乙酸作为沉淀剂．以Symmetry C18（46mm×150mm，5um）为色谱柱，乙腈：水：3mmol·L^-1磷酸盐缓冲液（含0．1％庚烷磺酸钠和0．5％三乙醇胺。pH4．5）（7：43：50）为流动相，流速为1．0mL·min^-1，柱温30℃，检测波长为233nm。结果盐酸二甲双胍浓度在1～100μg·mL^-1范围内线性关系良好，回归方程为A=9．627416×10^4C．7．9609643×10^3（r=0．9998），平均回收率为99．21％。结论本法简单、快捷、灵敏、准确，适用于临床药代动力学及药效学的研究。

Determination of hydrochloride Metformin in human plasma by RP-HPLO

OBJECTIVE To develop a method for the determination of hydrochloride metformin in human plasma. METHODS Samples of plasma were precipitated with trichloroacetic acid. A Symmetry C18 column was used with a mobile phase of acetonitrile-water-phosphate buffer （containing 0.1% sodium beptanesulfonate, 0.5 % triethanolamine and adjusted pH to 4.5） （7： 43 ： 50）. The flow rate was 1.0mL· min^- 1, the temperature of the column was 30℃, the detection wavelength was at 233.0nm. RESULTS A good linearity was obtained from 1 to 100μg·mL^-1 plasma concentration of hydrochloride metformin A = 9. 627416 × 10^4C-7. 9609643 ×10^3 （r = 0. 9998）. The mean recovery was 99.21%. CONCLUSION The method was simple, rapid, accurate, and suitable for being applied to study of pharmacokinetics and pharmacodynamics of metformin hydrochloride in clinical.