东北鹤虱提取物抗腹泻作用机制的探讨

目的：观察东北鹤虱提取物（LEGA）对肠道平滑肌收缩活动的影响并探讨其抗腹泻作用机制。方法：以碳末推进率为测量指标，观察LEGA对小鼠小肠蠕动及大鼠结肠推进运动的影响；以大鼠十二指肠收缩强度为指标，观察LEGA对肠管平滑肌收缩运动的影响；采用Robert实验法观察LEGA对小肠水分吸收的影响。结果：LEGA可抑制小鼠小肠蠕动及大鼠结肠推进运动（P〈0．01），剂量依赖性地使大鼠十二指肠平滑肌自主收缩幅度下降（P〈0．01），拮抗ACh、5-HT、BaCl2诱导的十二指肠痉挛性收缩，还促进小肠内水分的吸收（P〈0．01）。结论：LEGA通过抑制肠道的过强运动、降低肠内压和调节水运动失调而缓解腹泻。

A study on the anti-diarrhea mechanism of abstract from Lappula Echinata Gilib

Objective： To study the effects and mechanism of abstract from Lappula Echinata Gilib （LEGA） on anti-diarrhea. Methods： The effects of LEGA on mice intestine and rat colon were observed with the movement rate of charcoal; the influence of LEGA on the intensity of rat duodenum were studied; the fluid and chloridion absorption in small intestine were also observed with the Robert method. Results： LEGA could inhibit the peristalsis of the intestine and the colon （P〈0.01）, decrease spontaneous contraction amplitude of smooth muscle dose-dependently （P〈0.01）, antagonize the contraction of rat duodenum induced by ACh, 5-HT, BaCl2, and improve the absorption of small intestinal juice （P〈0.01）. Conclusion： LEGA can relieve the diarrhea by inhibiting the spasmodic contraction of gut soomth muscle, decreasing the internal pressure and regulating the water balance of the intestine.