Nasal mucosal inhalation of amyloid-beta peptide 3–10 defective adenovirus attenuates cytotoxicity induced by beta-amyloid(1–42) |
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作者姓名: | Tongzi Jiang Wanshu Guo Sha Sha Xiaona Xing Rong Guo Yunpeng Cao |
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作者单位: | Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China |
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摘 要: | Three-month-old Alzheimer's disease model transgenic mice were immunized with Aβ1–42, Plp-Adenovirus Ad]-X-CMV-(Aβ3–10)10-CpG AdCpG-(Aβ3–10)10] or AdCpG virus fluid via nasal mucosal inhalation, respectively. ELISA analysis of serum showed Aβ42 antibody titers were significantly increased in mice immunized with Aβ1–42 and AdCpG-(Aβ3–10)10. Concanavalin A and AdCpG-(Aβ3–10)10 stimulation significantly increased the number of proliferating spleen cells cultured from AdCpG(Aβ3–10)10 and Aβ42 groups compared with the control group. In the AdCpG(Aβ3–10)10 group, levels of interleukin(IL)-4 and IL-10 were increased, while those of IL-2 and interferon-γ were decreased. In the Aβ42 group, levels of IL-4, IL-10, IL-2 and interferon-γ were all increased. Experimental findings indicate that AdCpG-(Aβ3–10)10 vaccine can produce strong T helper 2(Th2) humoral immune responses in addition to the production of Aβ42 antibody. The cellular immunologic response was weak and avoided Aβ1–42-mediated cytotoxicity.
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关 键 词: | β-淀粉样蛋白 腺病毒 酶联免疫吸附分析 缺陷型 阿尔茨海默氏症 伴刀豆球蛋白A 转基因小鼠 IL-10 |
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