新生血管性青光眼患者眼内液血管 内皮生长因子的检测

目的了解新生血管性青光眼(neovascular glaucoma, NVG)患者房水及玻璃体中血管内皮生长因子(vascular endothelial growth factor, VEGF)的含量以及对虹膜新生血管发生的影响。方法应用酶联免疫吸附测定法(enzyme linked immu nosorbent assay, ELISA)分别对11例NVG患者行内眼手术时抽取的房水、玻璃体22个标本以及同期6例黄斑裂孔手术患者(对照组)房水、玻璃体12个标本进行VEGF检测。结果NVG患者房水、玻璃体中VEGF含量分别为(1.451±0.247)、(1.610±0.125) ng/ml，较对照组房水、玻璃体中的 VEGF含量(0.189±0.038)、(0.201±0.055) ng/ml明显增高， 两组间差别有非常显著性意义(t=12.007，P<0.001；t=26.057，P<0.001)。结论NVG患者房水、玻璃体中VEGF浓度显著增高，提示VEGF在NVG患者虹膜新生血管形成过程中可能具有一定作用。(中华眼底病杂志,2001,17:305-306)

Vascular endothelial growth factor in ocular fluid of patients with neovascular glaucome

ObjectiveTo detect the levels of vascular endoth elial growth factor (VEGF) in aqueous humor and vitreous of patients with neovascular glaucoma (NVG) and infer their possible effect on the development of neovascularization of iris.MethodsThe concentration of VEGF in 22 samples of ocular fluid of aqueous humor and vitreous respectively obtained from 11 patients with NVG undergone intraocular surgery were measured by using enzyme linked immunosobent assay (ELISA) for quantitative analysis. As control, 12 samples of ocular fluid of 6 patients with macular hole were detected by the same methods.ResultsThe mean [AKx-]±s VEGF concentrations in aqueous humor and vitreous from patients with NVG were [(1.451±0.247)、(1.610±0.125) ng/ml] higher than those in the cotrol group [(0.189±0.038)、(0.201±0.055) ng/ml], there was a significant difference between the two groups statistically (t=12.007，P<0001；t=26.0 57，P<0.001).ConclusionThe patients with NVG have significantly increased level of VEGF in ocular fluid, and VEGF might fill the role in mediating active iris neovascularization.(Chin J Ocul Fundus Dis, 2001,17:305-306)