三氯乙烯对大鼠肝细胞DNA损伤和氧化应激的影响

目的研究三氯乙烯对大鼠肝细胞DNA损伤和肝组织氧化应激的作用。方法选用雄性SD大鼠，每组5只，用1500和3000mg／kg的三氯乙烯经口灌胃染毒，48h处死动物。用单细胞凝胶电泳（彗星实验）检测肝细胞DNA损伤，并检测肝组织中活性氧（ROS）、丙二醛（MDA）、还原型谷胱甘肽（GSH）的含量。结果三氯乙烯在1500和3000ms／ks剂量条件下，肝细胞DNA的Olive尾矩增加，DNA损伤细胞率分别为41．96％和54．13％，也明显高于对照组的9．30％（P〈0．01）；大鼠肝组织中的ROS、MDA均较对照组显著增高（P〈0．01），同时GSH又较对照组明显下降（P〈0．01）。结论三氯乙烯在1500和3000mg／kg剂量条件下染毒大鼠后，造成肝组织明显的氧化应激并引起肝细胞DNA损伤。

Effect of Trichloroethylene on DNA Damage and Oxidative Stress in Rat Hepatocytes

[ Objective ] To study the effects of triehloroethylene on DNA damage in rat hepatocytes and oxidative stress in rat liver tissue. [Methods] Male SD rats were selected, 5 for each group. Trichloroethylene at the doses of 1500 and 3000 mg/kg were poured into rats＇ stomach through mouth, then they were killed after 48 hours. DNA damage was detected by single-cell gel electro- phoresis. Contents of Reactive Oxygen Species （ ROS）, Malondialdehyde （ MDA ） and Glutathione （GSH） were also measured. [ Results ] Trichloroethylene at the doses of 1500 and 3000mg/kg caused DNA olive tail moment increased, DNA damage rate was 41.96% and 54.13%, respectively, which was significantly higher than that of control group （P 〈0.01 ） ; while GSH decreased signifantly compared with control group （P 〈 0.01）. [ Conclusion] Trichloroethylene at the doses of 1500 and 3000mg/kg induces obvious oxidative stress in rat liver tissue and causes DNA damage in rat hepatocytes.