| 中药提取物逆转K562/ADM细胞的耐药作用及其分子机制的探讨 |
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| 引用本文： | 乔高娟,李贵海,杨炜华,温培娥,任霞,范华,唐天华,任海全,姜国胜. 中药提取物逆转K562/ADM细胞的耐药作用及其分子机制的探讨[J]. 国际肿瘤学杂志, 2006, 36(1): 474-476. DOI: 10.3760/cma.j.issn.1673-422X.2009.06.026 |
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| 作者姓名： | 乔高娟 李贵海 杨炜华 温培娥 任霞 范华 唐天华 任海全 姜国胜 |
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| 作者单位： | 250062济南,山东省医学科学院基础医学研究所,山东省医药卫生肿瘤免疫与中药免疫莺点实验室;山东省中医药研究院; |
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| 基金项目： | 山东省自然科学基金山东省科技项目 |
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| 摘 要： | Objective To investigate the reversal effect of the monomer of traditional Chinese medicine on muhidrug resistance(MDR) and its possible mechanism in K562/ADM cell line in vitro. Methods With different concentrations of baicalin, geniposide administered to K562/ADM cells, the proliferation of K562/ ADM cells was detected by the MTY assay. Expression of mdr-1 mRNA, Topo Ⅱ mRNA was measured by semi-quantitive RT-PCR. Results Thatbaicalin and geniposide could increase the sensitivity of K562/ADM cells to adriamycin, multiples of reversion were 1.95 times and 1.46 times. The proliferation of K562/ADM cells was in-hibited obviously by baicalin and geniposide, the level of mdr-1 mRNA expression was down-regulated and the Topo Ⅱ mRNA was up-regulated(P<0.01 ). Conclusion Baicalin and geniposide may reverse the multi-drag-resistance of K562/ADM cells, which was related to the down-regulation of mdr-1 expression and up-reg-ulation of Topo Ⅱ beta expression.
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| 关 键 词： | 黄芩 栀子 P糖蛋白 白血病 |
Reversal of multidrug resistance of K562/ADM cells in vitro by the monomer of traditional Chinese medicine extract and investigation of its molecular mechanism |
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| QIAO Gao-juan,LI Gui-hai,YANG Wei-hua,WEN Pei-e,REN Xia,FAN Hua,TANG Tian-hua,REN Hai-quan,JIANG Guo-sheng. Reversal of multidrug resistance of K562/ADM cells in vitro by the monomer of traditional Chinese medicine extract and investigation of its molecular mechanism[J]. Journal of International Oncology, 2006, 36(1): 474-476. DOI: 10.3760/cma.j.issn.1673-422X.2009.06.026 |
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| Authors: | QIAO Gao-juan LI Gui-hai YANG Wei-hua WEN Pei-e REN Xia FAN Hua TANG Tian-hua REN Hai-quan JIANG Guo-sheng |
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| Abstract: | |
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| Keywords: | Scutellaria baicalensisGardenia jasminoidesP-glycoproteinLeukemia |
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