葡萄膜炎患者血清干扰素-γ、肿瘤坏死因子-α和白细胞介素-6水平的检测及意义

目的 观察急性葡萄膜炎患者治疗前后血清干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）水平变化，探讨其在急性葡萄膜炎发生发展过程中的作用。方法 连续收集各类急性葡萄膜炎患者75例，用酶联免疫吸附试验测定其急性期和治疗后恢复期血清IFN-γ、TNF-α和IL-6水平，并与同期收集的30例健康人血清IFN-γ、TNF-α和IL-6检测结果进行比较。结果 葡萄膜炎患者急性期血清IFN-γ、 TNF-α和IL-6水平明显高于治疗恢复期和对照者（F=65.805、50.418、155.381，P=0.000），并且与其初诊时视力呈明显负相关（r=-0.656、-0.592、-0.653，P=0.000），3种细胞因子之间呈正相关（r=0.340、0.467、0.338，P＜0.05）。结论 INF-γ、TNF-α和IL-6在急性葡萄膜炎患者血清中明显升高，治疗后均下降，表明这些细胞因子参与了葡萄膜炎的发生和发展过程。

Measurement and significance of serum interferon-γ,tumor necrosis factor-α and interleukin-6 in patients with uveitis

Objective To examine the levels of interferon-γ(INF-γ).tumor necrosis factor-α (TNF-α)and interleukin-6(IL-6)in serum of patients with acute uveitis before and after treatment,and to explore the possible roles of those cytokines in the initiation and progression of the u,ieitis. Methods A series of 75 patients with acute uveitis.and 30 healthy persons from our hospital were investigated.The Ievels of INF-γ,TNF-α and IL-6 in acute phase and convalescent phase were measured by the enzymelinked immunosorbent assay. Result The serum 1evels of INF-γ,TNF-α and IL-6 in acute phase were significantly higher than that of the convalescent phase and the healthy controls(F=65.805/50.418/155.381,P=0.000).A significant negative correlation was found between the serum leveis of INF-γ,TNF-α and IL-6 in acute phase with their initiaI visual acuity(r=-0.656,-0.592 and-0.653,P＜O.01).There was also a positive correlation among the serum levels of INF-γ,TNF-α and IL-6(r=0.340,0.467 and 0.338,P＜0.05). Conclusions There are high serum levels of INF-γ,TNF-α and IL-6 in patients with acute uveitis,and the cytokines levels were decreased after the treatment.The results suggested that the INF-γ,TNF-α and IL-6 involved in initiation and progression of uveitis.