肠易激综合征患者肠粘膜紧密连接蛋白claudin-1,-3,-4表达改变的意义

目的 探讨claudin-1,-3,-4蛋白的改变在不同类型肠易激综合征患者发病中的作用.方法 随机选择肠易激综合征患者43例分为腹泻型组23例、便秘型组20例,对照组20例,用Western blotting方法分别检测各组肠粘膜claudin-1,-3,-4蛋白含量.结果 与对照组相比,小肠和结肠粘膜claudin-1蛋白含量在便秘型组均明显上升(P＜0.05).在腹泻型组均明显下降(P＜0.05);claudin-3在便秘型组明显上升(P＜0.05),在腹泻型组无明显的变化(P＞0.05),claudin-4变化跟claudin-1一致.结论 claudin-1,-4蛋白表达下降可能与腹泻型肠易激综合征的发病有关,而claudin-1,-3,-4蛋白表达的升高可能与便秘型肠易激综合征的排便性状和习惯改变有关.

Changes of tight junction claudin-1,-3,-4 protein expression in the intestinal mucosa in patients with irritable bowel syndrome

Objective To investigate the changes of intestinal mucosal tight junction proteins claudin-1,-3,-4 in patients with irritable bowel syndrome (IBS), and elucidate its possible role in the bowel evacuation habit changes and formation in these patients. Methods Western blotting was employed to determine tight junction protein claudin-1,-3,-4 levels in the intestinal mucosa of patients in the control group, diarrhea-predominant IBS (D-IBS) group and constipation-predominant IBS (C-IBS) group. Results Compared with the control group, D-IBS patients showed significantly decreased claudin-1 protein levels in both the small intestinal and colonic mucosae (P<0.05), whereas C-IBS patients had significantly elevated claudin-1 protein levels (P<0.05). No significant difference was found in claudin-3 protein expression in the both small intestinal and colonic mucosae between the D-IBS group and the control group (P>0.05), but claudin-3 protein level was shown to increase significantly in C-IBS patients (P<0.05). Claudin-4 protein followed the same pattern of alteration as claudin-1. Conclusion Down-regulated claudin-1 and-4 expressions can be associated with bowel evacuation habit changes and formation in patients with D-IBS, but up-regulated claudin-1,-3 and-4 expressions may relate to such bowel changes in patients with C-IBS.