磷素对怀牛膝耐铜毒性的影响研究

目的:通过施磷(P)来调控怀牛膝对铜(Cu)的吸收和累积,防止怀牛膝过量吸收重金属Cu.方法:采用2因素完全设计,用自制PVC管栽培.结果与结论:Cu处理间、P处理间以及Cu,P交互间怀牛膝生物量差异都达到显著水平,土壤中P,Cu均为100 mg·kg~(-1)时生物量最大;低Cu可以增加怀牛膝产量,Cu超过200 mg·kg~(-1)后,怀牛膝产量、质量均下降,但对药材中活性成分齐墩果酸和脱皮甾酮的含量无影响.P的施入可以减轻高Cu对植株的毒害,怀牛膝产量增加,重金属Cu含量降低,但是当土壤Cu超过200 mg·kg~(-1)后,怀牛膝药材中Cu高于20.0.mg·kg~(-1)的限度,因此要生产出合格的怀牛膝药材,种植基地土壤中Cu一定要低于200 mg·kg~(-1).

Effect of phosphorus on copper tolerance in Achyranthes bidentata

Objective: To study the effect of phosphorus on copper tolerance in Achyranthes bidentata. Method: A PVC pipe experiment was conducted to study the interactive effects of phosphorus (P) and copper (Cu), on growth, elemental accumulation and chemical constituents of A. bidentata. Two levels of elemental P were applied at 0 (P0) and 100 (P100) mg·kg-1 soil with 5 levels of Cu at 0 (Cu0), 100 (Cu100), 200(Cu200), 200(Cu400), 200(Cu600) mg·kg-1 soil, respectively. Result and Conclusion: The biomass production between different Cu treatments, phosphorus treatment showed significant differences. The biomass reached the maximum value as the concentration of Cu and P was 100 mg·kg-1. Low concentration of Cu improved the growth of A. bidentata. The growth was blocked as Cu concentration reached 200 mg·kg-1 in soil, however the contents of oleanolic acid and ecdysterone in roots of A. bidentata had not influenced by Cu. P could improved the copper tolerance in A. bidentata and increased root yield. The Cu concentration in soil of the cultivation bases must be below 200 mg·kg-1 in order to produce good quality of medicinal material.