大鼠实验性视网膜光损伤中的视细胞凋亡

目的 进一步探讨视网膜光损伤的发病机制。 方法 20只Wistar大鼠分为实验组、对照组，分别在光照后12，24，36小时摘除眼球，视网膜组织行HE染色和核苷酸末端转移酶介导的DUTP缺口翻译法(TdT-mediated dUTP nick end labelling method,TUNEL)标记凋亡细胞。 结果 光照后12小时，视杆细胞外节出现少量空泡变性；24小时后，外核层出现明显的细胞核破碎、浓染和DNA裂解；36小时后，视杆细胞内、外节溶解，外核层大量细胞核丢失。 结论 视细胞凋亡是大鼠实验性视网膜光损伤的重要机制之一。 (中华眼底病杂志, 1999, 15: 167-169)

Apoptosis of photoreceptor cell in experimental rat retinal photoinjury

Objective To further investigate pathologic mechanism of retinal phototrauma. Methods Twenty Wistar rats were divided into control and experimental groups.Their eyes were extracted in 12,24 and 36 hours after light exposure.HE stained retina samples were examined and TDT mediated dUTP nick end labelling(TUNEL)method was employed to distinguish apoptotic cells. Results After 12 hour light exposure,slight vesiculation was observed in the rod outer segment of the retinas.After 24 hour light exposure,the outer nuclear layer showed predominant fractured and condensed nuclei and fragmented DNA.After 36 hour light exposure,the rod outer and inner segments were lysed and most of the nuclei in the outer nuclear layer were disappeared. Conclusions Apoptosis of photoreceptor cell is one of the important mechanisms which cause experimental retinal photoinjury of rats.