系统性红斑狼疮患者血清抗核小体抗体检测的意义

目的 :探讨血清抗核小体抗体 (ANuA)的检测在系统性红斑狼疮患者中的意义。方法 :采用酶联免疫吸附试验检测5 8例SLE患者 ,4 3例其它结缔组织病患者 ,2 0例非结缔组织病患者及 2 0例健康人血清抗核小体抗体。结果 :SLE患者血清ANuA阳性率为 74 .1%。SLE患者与健康对照组血清中ANuA阳性率差别具有高度显著性 (P <0 .0 0 1) ,而其它结缔组织病和非结缔组织病与健康对照组血清中ANuA阳性率差别均无显著性 (P >0 .0 5 )。SLE活动期患者与非活动期患者ANuA阳性率差别具有高度显著性 (P <0 .0 0 1)。结论 :ANuA可以作为除抗dsDNA和抗Sm以外的诊断SLE和判断SLE活动期的一种新的自身抗体。

Significance of anti- nucleosome antibodies in systemic lupus erythematosus

Objective: To study the clinical significance of determining serum anti-nucleosome antibodies(ANuA) in systemic lupus erythematosus patients(SLE). Methods: ANuA were detected by enzyme-linked immunoadsordent assay(ELISA) in the serum of 58 SLE patients,43 patients with different connective diseases(CTD) except SLE,20 patients with non-CTD and 20 normal subjects.Results: The positive rate of ANuA in SLE is 74.1%.Compared with normal,the positive rate of ANuA in the groups of CTD except SLE and the groups of non-CTD was not significantly different( P >0.05) and there was significant difference between the groups of SLE and normal ( P< 0.001).The positive rate of ANuA was significantly different between the active SLE and the non-active SLE. Conclusion: Besides anti-dsDNA and anti-Sm,anti-nucleosome antibodies may be a useful marker for diagnosis and activity assessment of SLE.