| 内皮祖细胞的鉴定及培养方法 |
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| 引用本文： | 李玉娟,刘建勋. 内皮祖细胞的鉴定及培养方法[J]. 中国药理学通报, 2009, 25(10) |
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| 作者姓名： | 李玉娟 刘建勋 |
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| 作者单位： | 中国中医科学院西苑医院实验研究中心,北京,100091 |
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| 摘 要： | 近10年来,内皮祖细胞(endothelial progenitor cells,EPCs)的研究受到越来越多的关注。研究发现,循环血液中EPCs储量的改变伴随着心血管疾病的不同表现。因此,EPCs作为心血管疾病的生物标志物及修复受损血管的一种细胞疗法被广泛研究。该文对1997年至今大多数研究中EPCs的鉴定及培养方法做了简要综述。
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| 关 键 词： | 内皮祖细胞 细胞培养 流式细胞术 血管新生 血管发生 |
The identification and culture methods of endothelial progenitor cells |
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| LI Yu-juan,LIU Jian-xun. The identification and culture methods of endothelial progenitor cells[J]. Chinese Pharmacological Bulletin, 2009, 25(10) |
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| Authors: | LI Yu-juan LIU Jian-xun |
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| Abstract: | In the last 10 years,an increasing interest has been devoted to the study of endothelial progenitor cells(EPCs).Alteration and/or reduction of the circulating EPCs have/has been associated with different manifestation of cardiovascular disorders.Thererfore,EPCs have been extensively studied as biomarkers of cardiovascular disease and as a cell-based therapy for repair of damaged blood vessels.In the review,we focus on two methodologies used to identify and enumerate EPCs in the majority of studies up to date. |
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| Keywords: | endothelial progenitor cells,cell culture,flow cytometry angiogenesis vasculogenesis |
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