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SPE-HPLC法测定止嗽立效丸中吗啡的含量
引用本文:戴敬,冯丽,王璐,刘秀霞. SPE-HPLC法测定止嗽立效丸中吗啡的含量[J]. 中国药品标准, 2011, 12(3): 181-184
作者姓名:戴敬  冯丽  王璐  刘秀霞
作者单位:河北省药品检验检所,石家庄,050011
摘    要:目的:建立HPLC法测定止嗽立效丸中吗啡的含量。方法:预处理应用固相萃取技术;液相色谱采用Shim-pack C18柱(6.0 mm×150 mm,5μm),流动相为乙腈-0.05 mol.L-1磷酸二氢钾-0.005 mol.L-1庚烷磺酸钠(10∶45∶45),流速1 mL.min-1,柱温25℃,检测波长210 nm。结果:吗啡进样量在0.026×10~2.58μg范围内线性关系良好(r=0.999 9),平均回收率为101.3%,RSD为1.2%(n=9)。结论:方法简便、准确,重复性好。

关 键 词:固相萃取  高效液相色谱法  止嗽立效丸  吗啡

Determination of Morphine in Zhisou Lixiao Pills by SPE-HPLC
Dai Jing,Feng Li,Wang Lu and Liu Xiu-xia. Determination of Morphine in Zhisou Lixiao Pills by SPE-HPLC[J]. , 2011, 12(3): 181-184
Authors:Dai Jing  Feng Li  Wang Lu  Liu Xiu-xia
Affiliation:Dai Jing,Feng Li,Wang Lu,Liu Xiu-xia(Hebei Provincial Institute for Drug Control,Shijiazhuang 050011)
Abstract:Objective: To develop an HPLC method for the determination of Morphine in Zhisou Lixiao Pills. Methods: Solid-phase extraction(SPE) was used in pretreatment. Shim-pack C18 column (6.0 mm × 150 mm, 5 μm) was used in HPLC. The mobile phase consisted of acetonitrile - 0.05 mol · L ^- 1 potassium dihydrogen phosphate solution - 0. 005 mol · L ^- 1 1-heptane sulfonic acid sodium salt solution ( 10: 45:45 ). The flow rate was 1 mL· min^-1 , and morphine was detected by UV absorption at 210 nm. Results:The calibration curve was linear in the range of 2. 6 × 10^-2 -2. 58 μg (r =0. 999 9). The average recovery was 101.3% , and RSD was 1.2% (n =9 ). Conclusion:This method is simple and accurate with good repeatability.
Keywords:SPE(solid-phase extraction)  HPLC  Zhisou Lixiao Pills  Morphine  
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