| Ca2+/PKC通路在大鼠缺氧复氧心肌细胞Egr-1高表达中的作用 |
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| 引用本文： | 郑付春,贾强用,石刚刚. Ca2+/PKC通路在大鼠缺氧复氧心肌细胞Egr-1高表达中的作用[J]. 中国临床药理学与治疗学, 2011, 16(3): 241-248 |
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| 作者姓名： | 郑付春 贾强用 石刚刚 |
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| 作者单位： | 1. 汕头大学医学院第一附属医院药剂科
2. 汕头大学医学院药理教研室,汕头,515041,广东 |
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| 基金项目： | 国家自然科学基金-广东联合基金，中国高等教育博士点研究基金 |
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| 摘 要： | 目的:观察Ca2+/PKC通路在大鼠心肌细胞缺氧复氧状态下参与Egr-1高表达。方法:取生长5~6 d的大鼠心肌细胞分为对照(Control)组、缺氧复氧(H/R)组、溶剂(DMSO)组、EGTA组、维拉帕米(VER)组、H7组和BIM组。各组心肌细胞制作H/R模型。RT-PCR法检测培养心肌细胞中Egr-1 mRNA的表达水平。Western-blot法检测培养心肌细胞中Egr-1蛋白的表达水平。结果:与Control组相比,H/R组及DMSO组心肌细胞Egr-1 mRNA的表达水平明显增高(P<0.05);与H/R组相比,VER组、EGTA组、H7组、BIM组Egr-1 mRNA的表达明显下调,差异具有统计学意义(P<0.05)。与Control组相比,H/R组及DMSO组心肌细胞Egr-1蛋白表达水平明显增高(P<0.05);与H/R组相比,VER组、EGTA组、H7组、BIM组Egr-1蛋白的表达明显下调,差异具有统计学意义(P<0.05)。结论:在大鼠心肌细胞缺氧复氧状态下,Ca2+/PKC信号通路参与介导了Egr-1的高表达。
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| 关 键 词： | Ca2+ 蛋白激酶C Egr-1 心肌细胞 缺氧复氧 |
Effects of Ca2+/PKC pathway on Egr-1 overexpression in hypoxia/ reoxygenation cardiomyocytes of rats |
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| ZHENG Fu-chun,JIA Qiang-yong,SHI Gang-gang. Effects of Ca2+/PKC pathway on Egr-1 overexpression in hypoxia/ reoxygenation cardiomyocytes of rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(3): 241-248 |
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| Authors: | ZHENG Fu-chun JIA Qiang-yong SHI Gang-gang |
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| Affiliation: | ZHENG Fu-chun1,JIA Qiang-yong2,SHI Gang-gang21 Department of Pharmacy,1st Affiliated Hospital,Shantou University Medical College,2 Department of Pharmacology,Shantou 515041,Guangdong,China |
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| Abstract: | AIM: To study effects of Ca2+/PKC pathway on Egr-1 overexpression in hypoxia/reoxygenation cardiomyocytes of rats.METHODS: Cultured cardiomyocytes of 5-6 day old rats were divided into control group,hypoxia/reoxygenation(H/R) group,DMSO group,EGTA group,Verapamil group,H7 group and BIM group.All groups of cardiomyocytes underwent the treatment of H/R,and then RT-PCR and Western blot were used to test expression of Egr-1 mRNA and Egr-1 protein respectively.RESULTS:There were high expression of Egr-1 mRNA in ... |
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| Keywords: | Ca2+ Egr-1 |
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