凝胶色谱法测定普鲁卡因青霉素中的高分子聚合物 |
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引用本文: | 殷果,秦斌,邓颖,仇士林.凝胶色谱法测定普鲁卡因青霉素中的高分子聚合物[J].中国药品标准,2011,12(3):178-181. |
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作者姓名: | 殷果 秦斌 邓颖 仇士林 |
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作者单位: | 1. 深圳市药品检验所,深圳,518029 2. 上海市食品药品检验所,上海,200030 |
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摘 要: | 目的:建立普鲁卡因青霉素中高分子聚合物测定方法。方法:采用凝胶色谱法。色谱柱为Sephadex G-10(40~120μm)凝胶柱,流动相A为0.03 mol.L-1磷酸盐缓冲液(pH 7.0),流动相B为超纯水,流速1.5 mL.min-1;检测波长254 nm。结果:普鲁卡因青霉素的进样浓度在10~50 mg.mL-1的范围内与青霉素聚合物的峰面积呈良好线性关系(r=0.999 6)。结论:该方法灵敏、准确、简便,可用于本品的质量控制。
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关 键 词: | 凝胶色谱法 普鲁卡因青霉素 高分子杂质 |
Determination of the High Molecular Weight Impurities in Procaine Benzylpenicillin by Gel Filtration Chromatography |
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Authors: | Yin Guo Qin Bin Deng Ying and Qiu Shi-lin |
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Institution: | Yin Guo1,Qin Bin1,Deng Ying1,Qiu Shi-lin2(1.Shenzhen Institute for Drug Control,Shenzhen 518029,2.Shanghai Institute for Food and Drug Control,Shanghai 200030) |
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Abstract: | Objective: To establish a method for determination of high molecular impurities in Procaine Benzylpenicillin. Method : Gel filtration chromatography was adopted. The analytical column was Sephadex G-10 gel column; moblie phase A: 0. 03 mol · L^-1 phos- phate buffer( pH 7.0) ; moblie phase B : pure water; the flow rate : 1.5ml . min ; the wavelength : 254nm . Results: The calibration curve was linear in the concentration range of 10 - 50 mg · mL^-1 (r = 0. 999 6). Conclusion: The method is sensitive, accurate, simple, and conrol of Procaine Benzylpenicillin. |
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Keywords: | Gel filtration chromatography Procaine Benzylpenicillin High-molecular weight impurity |
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