雷公藤多甙对胶原诱导性关节炎大鼠IL-10表达的影响

目的研究雷公藤多甙对类风湿关节炎大鼠的作用,探讨类风湿关节炎的发病机制及雷公藤多甙的作用机制,为临床应用提供理论基础。方法本实验应用鸡Ⅱ型胶原蛋白,建立胶原诱导性关节炎(collagen-induced arthritis,CIA)大鼠模型。应用酶联免疫吸附试验方法(ELISA)检测观察正常对照组、CIA模型组和雷公藤多甙治疗组大鼠血液及关节液中白细胞介素-10(interleukin-10,IL-10)的浓度。结果 ELISA检测结果显示,类风湿关节炎大鼠血清和关节液中IL-10含量明显减低,而雷公藤多甙可以升高IL-10的浓度,使之趋向正常。结论雷公藤多甙可有效减轻佐剂型关节炎大鼠的关节病变,并能使CIA大鼠血清、关节浸液IL-10分子水平增高。

The effect of Tripterygium Glycosides on the expression of IL-10 in collagen-induced arthritis rat

Objective To investigate the therapeutic effect of Tripterygium Glycosides(TG) on rheumatoid arthritis(RA) rat model,explore the pathogenesis of RA,and study the pharmaco-mechanism of Tripterygium Glycosides.The study could provide experimental datas for TG clinical use on RA.Methods The chicken II collagen was used to induce RA to established collagen-induced arthritis(CIA) rat model.The levels of IL-10 in rat's blood and synovial fluid were determined using Enzyme-linked immunoassay method(ELISA).Results The mass of lymphocytes of CIA groups were significantly higher than normal group(P<0.05).TG treated group has similar mass lymphocytes to normal group.ELISA results indicated that the concentration of IL-10 in serum and synovial fluid of CIA group were significantly lower than that in normal group.However,the concentration of IL-10 could be improved to normal level by applying TG.Conclusion TG could potentially alleviate articular damage of adjuvant induced arthritis(AA) rats,TG also could improved the concentration of IL-10 in serum and synovial fluid.