| 负压与BMSCs膜片促进细胞定植和体内成骨的探讨 |
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| 引用本文： | 温鑫鑫,周鹂鹂,刘宏伟.负压与BMSCs膜片促进细胞定植和体内成骨的探讨[J].口腔颌面外科杂志,2013,23(2):85-89. |
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| 作者姓名： | 温鑫鑫 周鹂鹂 刘宏伟 |
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| 作者单位： | 温鑫鑫 (同济大学附属口腔医院牙周科,同济大学口腔医学院口腔生物医学及转化医学实验室,上海200092)；周鹂鹂 (同济大学附属口腔医院牙周科,同济大学口腔医学院口腔生物医学及转化医学实验室,上海200092)； 刘宏伟 (同济大学附属口腔医院牙周科,同济大学口腔医学院口腔生物医学及转化医学实验室,上海200092)； |
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| 基金项目： | 国家自然科学基金资助项目(81271152);上海市科委科技计划项目(074119514) |
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| 摘 要： | 目的:研究经过负压处理的TCP支架材料与细胞复合后的体内成骨能力。方法:将TCP支架材料负压处理,和骨髓基质细胞(BMSCs)细胞共培养一段时间后,成骨诱导2周,电镜下观察支架材料和细胞复合情况。再将该复合物与BMSCS细胞膜片复合,植入到裸鼠皮下,8周后作组织切片,HE染色和Masson染色,观察其体内成骨能力。结果:负压(有细胞)+膜片组异位移植8周后,内部可见大量胶原纤维和成骨纤维,在团块样组织内部可见大量成骨细胞,并有血管形成。对照组仅有个别细胞密集区,胶原纤维和成骨纤维较少。空白组未见类似情况。结论:经过负压+骨髓基质细胞膜片处理的TCP支架材料,在体内有成骨潜能。
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| 关 键 词： | 组织工程 骨再生 骨髓基质细胞 细胞膜片 负压 |
Application of Negative Pressure System and BMSCs Cell Sheet in Bone Formation in vivo |
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| WEN Xin-xin,ZHOU Li-li,LIU Hong-wei.Application of Negative Pressure System and BMSCs Cell Sheet in Bone Formation in vivo[J].Chinese Journal of Oral and Maxillofacial Surgery,2013,23(2):85-89. |
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| Authors: | WEN Xin-xin ZHOU Li-li LIU Hong-wei |
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| Institution: | (Department of Periodontology,Hospital of Stomatology,Laboratory of Oral Biomedical Science and Translation Medicine,School of Stomatology,Tongji University,Shanghai 200072,China) |
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| Abstract: | Objective:The present study was proposed to investigate in vivo osteogenic potential of bone marrow stromal cells(BMSCs) in negative pressure treated β-Tricalcium phosphate(β-TCP) scaffolds.Methods:TCP was dealt with the syringe system(made for a subatmospheric pressure),cocultured or not cocultured with BMSCs,and differentiate into osteogenic direction for 2 weeks.BMSCs sheet was packed into TCP scaffolds and embedded subcutaneously in nude mice for 8 weeks.Animals were sacrificed,osteogenic potential were observed histologically by HE,and Masson stain.Results:TCP cocultured with BMSCs packed with cell sheets showed better osteogenic potential in vivo.Conclusion:Because of the formation of extracellular matrix,the negative pressure and cell sheets may promote cell colonization and improve bone formation in vivo. |
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| Keywords: | tissue engineering bone regeneration bone marrow stromal cells cell sheet negative pressure system |
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