葡萄籽原花青素对血小板聚集与实验性血栓形成的影响

目的 观察葡萄籽原花青素对血小板聚集和实验性血栓形成的影响.方法 ①大鼠血栓形成实验按随机数字表法分为5组:生理盐水(对照)组、阿司匹林(ASP)组(60 mg/kg)及原花青素100 mg/kg、200 mg/kg、400 mg/kg组,每组各10只.②小鼠血栓形成实验按随机数字表法分为5组,生理盐水(对照)组、阿司匹林组(84mg/kg)及原花青素140mg/kg、280mg/kg、560mg/kg组,各10只.实验动物采用二磷酸腺苷(ADP)、胶原(COL)、花生四烯酸(AA)灌胃,诱导大鼠血小板聚集;采用大鼠动-静脉旁路丝线上血检形成和胶原蛋白-肾上腺素诱发小鼠体内血栓形成,两种方法均以阿司匹林为阳性对照.结果 ①原花肯素高、中、低剂量组均可抑制大鼠颈总动脉-颈外静脉血流旁路血栓的湿重:高、中剂量对血栓干重有明显作用,但不及阿司匹林.②原花青素高剂量组可对抗胶原蛋白-肾上腺素混合诱导剂引起的血栓形成,减少动物死亡数.⑨原花青素中、高剂量组均可抑制ADP、COL、AA诱导的大鼠血小板聚集及两种方法引起的实验性血栓形成.结论 原花青素具有抑制实验性血栓形成和抗血小板聚集作用.

Effect of procyanidins from grape seeds on platelet aggregation and the form of experimental thrombosis

Objective To observe the effect of grape seed Procyanidins on platelet aggregation and experimental thrombosis in rats.Methods 50 Experimental thrombosis male SD rats of clean grade,weighing 250～300 g,were randomly divided into five groups,normal saline(control)group,aspirin(ASP)group(60 mg/kg),and procyanidins 1 00 mg/kg,200 mg/kg,400 mg/kg group,10 in each.50 experimental thrombosis male Kunming mice of clean grade,weighing 18～20 g,were also randomly divided into five groups,normal saline(control)group,aspirin group(84 mg/kg)and Procyanidins 140 mg/kg,280 mg/kg,560 mg/kg group,10 in each.Intragastric administration was performed in these experimental animals.The effects of Procyanidins were observed on rat platelet aggregation induced by ADP,COL and AA.Experimental rat's thrombosis was induced by method of"arterio-venous"shunt and the death rate within 15 min was determined with collagen plus adrenaline induced thrombus model in mice,aspirin as a positive control.Results Procyanidins in the middle and high-dose group can inhibit ADP,COL,AA-induced platelet aggregation and experimental thrombosis obviously.Conclusion Procyanidins can inhibit experimental thrombosis and have anti-platelet aggregation effect.