Antibodies against terminal galactosyl α(1–3) galactose epitopes in patients with idiopathic myelofibrosis

Summary Sera from patients with myelofibrosis were analysed for circulating antibodies against an antigenic determinant characterized by two molecules of galactose in α 1–3 linkage (anti-Gal antibodies).
500% of patients were found to have values above the 90th percentile of the values of control sera chosen as a cut-off. The median level of the antibodies was significantly higher than the value detected in normal controls, but no difference coulti be found between patients with idiopathic myelofibrosis and those with myelofibrosis associated to a chronic myeloproliferative disorder.
Anti-Gal antibodies were found to correlate with disease activity and with platelet count whereas no correlation was detected with other haematological parameters.
Furthermore, for evaluation of disease activity, determination of serum anti-Gal antibodies was a sensitive and specific parameter.
We conclude that humoral immunity against Galα1–3Gal may provide a sensitive tool to detect disease activity in patients with idiopathic myelofibrosis and may be important for understanding its pathogenesis.