OK-432联合IL-2对C57BL/6小鼠Lewis肺癌抑制的实验研究

目的 研究ＯＫ－４３２与ＩＬ－２联用，抑制近交系小鼠Ｃ５７ＢＬ／６Ｌｅｗｉｓ肺癌的作用。方法 Ｃ５７ＢＬ／６小鼠Ｌｅｗｉｓ肺癌为模型，观察ＯＫ－４３２联合ＩＬ－２对肿瘤发生、生长、转移的影响，及体内对肿瘤细胞的作用。结果 二者联用对肿瘤体积和重量的抑制作用明显增强（Ｐ〈０．０１），增强抑瘤率（Ｐ〈０．０５），肺转移灶的数目明显减少。光镜下显示肿瘤灶中出现许多凋亡细胞；肿瘤组织电镜观察，瘤细胞核染色

The Experimental Study of OK-432 and IL-2 Combination Inhibition of C57BL/6 Mice Bearing Lewis Lung Cancer

Objective:To study the anti-tumor effect of combination OK-432 and IL-2 therapy on inbred C57BL/6 mice bearing Lewis lung cancer (LLC). Methods: As C57BL/6 mice bearing LLC model,OK-432 and IL-2 combination effects were observed on tumor occurrence, growth, metastasis, and tumor cell changes under light and electron microscopes in vivo. Results: Combination administration group significantly enhanced inhibition effects on tumor volume and weight versus control (P<0.01), increased inhibition rate(P< 0.05), and distinctly reduced the number of pulmonary metastasis foci. Under electron microscope,tumor cell emerged karyopyknosis,new-moon shaped or circular,nucleus membrane was clear,endoplas-mic reticulum was dilated,and mitochondrion was swollen. A lot of apoptosis cells emerged in the tumor foci under light microscope. Conclusion: Under the half-dose of them,combination therapy still had strengthened effects of inhibition on tumor without IL-2 ill-effect. The process of killing tumor was mainly to attack tumor cells directly,improving some kinds of cell interactions and inducing tumor cell apoptosis.