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A microarray-based method for detecting methylated loci |
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| Authors: | Hatada Izuho Kato Azusa Morita Sumiyo Obata Yayoi Nagaoka Kayuri Sakurada Akira Sato Masami Horii Akira Tsujimoto Atsumi Matsubara Kenichi |
| Institution: | (1) Gene Research Center, Gunma University, 3-39-22 Showa-machi, Maebashi 371-8511, Japan Tel. +81-27-220-8057; Fax +81-27-220-8059 e-mail: ihatada@showa.gunma-u.ac.jp, JP;(2) DNA Chip Research Inc., Yokohama, Japan, JP;(3) Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan, JP;(4) Department of Molecular Pathology, Tohoku University School of Medicine, Sendai, Japan, JP;(5) Taisho Laboratory of Functional Genomics, Nara Institute of Science and Technology, Ikoma, Japan, JP |
| Abstract: | CpG island DNA methylation plays an important role in regulating gene expression in development and carcinogenesis. We developed a new microarray-based method called methylation amplification DNA chip (MAD) for detecting differences in methylation. In this method, only methylated CpG islands from the two samples that we wanted to compare were amplified and used for hybridization. The resource material for the microarray was derived from the methylated DNA library of the sample in which we wanted to detect hypermethylation. Choosing the methylated DNA library as the resource material of the microarray increased the percentage of DNA fragments derived from hypermethylated loci on the microarray. Received: March 20, 2002 / Accepted: April 23, 2002 |
| Keywords: | DNA methylation DNA chip Microarray CpG island Hypermethylation |
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