| 初诊急性髓系白血病患者来源的骨髓间充质干细胞成骨分化能力增强 |
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| 引用本文： | 宁红梅,王军,苏永锋,徐晨,扈江伟,楼晓,李秀森,毛宁,陈虎.初诊急性髓系白血病患者来源的骨髓间充质干细胞成骨分化能力增强[J].中国实验血液学杂志,2019(4):1277-1286. |
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| 作者姓名： | 宁红梅 王军 苏永锋 徐晨 扈江伟 楼晓 李秀森 毛宁 陈虎 |
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| 作者单位： | 解放军总医院第五医学中心造血干细胞移植科;军事科学院军事医学研究院军事认知与脑科学研究所 |
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| 基金项目： | 国家自然科学基金项目(81070435) |
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| 摘 要： | 目的:探讨急性髓系白血病(AML)患者骨髓微环境对间充质干细胞(MSC)生物学特性的影响。方法:从初诊的AML患者骨髓中分离得到MSC,在倒置显微镜下观察细胞形态,采用流式细胞术测定其免疫学表型,MTT法检测其增殖能力,以成骨、成脂肪和成软骨诱导检测MSC的多向分化能力。结果:从初诊AML病患者骨髓中分离得到的MSC呈现出经典的成纤维细胞样形态;其细胞表面高表达CD29,CD44,CD73,CD105和HLA-ABC,低表达CD14,CD31,CD34,CD45,CD80,CD86和HLA-DR。与健康人骨髓MSC相比,其增殖能力未见显著差别,但是其成骨细胞分化的能力显著增强。结论:初诊的AML患者骨髓中存在的MSC具有与健康人骨髓MSC相似的细胞形态、免疫表型和增殖能力,但是其成骨分化能力显著增强。
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| 关 键 词： | 初诊急性髓系白血病 间充质干细胞 成骨分化 |
Bone Marrow Mesenchymal Stem Cells from Newly Diagnosed Acute Myeloid Leukemia Patients Exhibit Enhanced Osteogenic Differentiation Capacity |
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| NING Hong-Mei,WANG Jun,SU Yong-Feng,XU Chen,HU Jiang-Wei,LOU Xiao,LI Xiu-Sen,MAO Ning,CHEN Hu.Bone Marrow Mesenchymal Stem Cells from Newly Diagnosed Acute Myeloid Leukemia Patients Exhibit Enhanced Osteogenic Differentiation Capacity[J].Journal of Experimental Hematology,2019(4):1277-1286. |
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| Authors: | NING Hong-Mei WANG Jun SU Yong-Feng XU Chen HU Jiang-Wei LOU Xiao LI Xiu-Sen MAO Ning CHEN Hu |
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| Institution: | (Department of Hematopoietic Stem Cell Transplantation,The Fifth Medical Center of Chinese PLA General Hospital,Beijing 100071,China;Institute of Military Cognition and Brain Sciences,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China) |
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| Abstract: | NING Hong-Mei;WANG Jun;SU Yong-Feng;XU Chen;HU Jiang-Wei;LOU Xiao;LI Xiu-Sen;MAO Ning;CHEN Hu(Department of Hematopoietic Stem Cell Transplantation,The Fifth Medical Center of Chinese PLA General Hospital,Beijing 100071,China;Institute of Military Cognition and Brain Sciences,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China) |
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| Keywords: | newly diagnosed acute myeloid leukemia mesenchymal stem cells osteogenic differentiation |
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