| GKT137831降低活性氧自由基水平来缓解阿托伐他汀所致的骨骼肌糖摄取下降 |
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| 引用本文： | 张荣,杨鑫泉,熊婷,唐姗,艾可青,钟红艳,毛永清,王大新. GKT137831降低活性氧自由基水平来缓解阿托伐他汀所致的骨骼肌糖摄取下降[J]. 实用临床医药杂志, 2019, 23(10): 53-57 |
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| 作者姓名： | 张荣 杨鑫泉 熊婷 唐姗 艾可青 钟红艳 毛永清 王大新 |
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| 作者单位： | 扬州大学医学院,江苏扬州,225001;中南大学湘雅医学院,湖南长沙,410000;大连医科大学,辽宁大连,116000 |
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| 基金项目： | 江苏省高层次人才培养工程"项目;江苏省"六大人才高峰"高层次人才项目 |
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| 摘 要： | 目的 探讨GKT137831对阿托伐他汀干预骨骼肌细胞的保护作用机制。 方法 采用阿托伐他汀干预骨骼肌C2C12细胞48 h。采用CCK-8方法分析阿托伐他汀对骨骼肌细胞生存率的影响。采用Western blot检测阿托伐他汀干预后NADPH氧化酶4(Nox4)蛋白表达。采用流式细胞术检测阿托伐他汀对骨骼肌细胞糖摄取及活性氧自由基(ROS)水平的影响。予Nox4抑制剂GKT137831共培养,分析阿托伐他汀对细胞糖摄取及ROS水平的影响。 结果 阿托伐他汀干预骨骼肌细胞48 h后, C2C12细胞糖摄取能力下降、ROS增加,予GKT137831后可缓解糖摄取下降及ROS增加。CCK-8实验结果显示,阿托伐他汀干预细胞48 h对细胞活力无影响。阿托伐他汀干预C2C12骨骼肌细胞后Nox4表达增加。 结论 GKT137831通过降低ROS水平来缓解阿托伐他汀所致的骨骼肌糖摄取下降。
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| 关 键 词： | GKT137831 阿托伐他汀 活性氧自由基 糖摄取 |
GKT137831 alleviates atorvastatin-induced decreased glucose uptake of skeletal muscle by suppressing reactive oxygen species level |
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| ZHANG Rong,YANG Xinquan,XIONG Ting,TANG Shan,AI Keqing,ZHONG Hongyan,MAO Yongqing,WANG Daxin. GKT137831 alleviates atorvastatin-induced decreased glucose uptake of skeletal muscle by suppressing reactive oxygen species level[J]. Journal of Clinical Medicine in Practice, 2019, 23(10): 53-57 |
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| Authors: | ZHANG Rong YANG Xinquan XIONG Ting TANG Shan AI Keqing ZHONG Hongyan MAO Yongqing WANG Daxin |
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| Affiliation: | (Clinical Medical College of Yangzhou University,Yangzhou,Jiangsu,225001;Xiangya Medical School of Central South University,Changsha,Hunan,410000;Dalian Medical University,Dalian,Liaoning,116000) |
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| Abstract: | ZHANG Rong;YANG Xinquan;XIONG Ting;TANG Shan;AI Keqing;ZHONG Hongyan;MAO Yongqing;WANG Daxin(Clinical Medical College of Yangzhou University,Yangzhou,Jiangsu,225001;Xiangya Medical School of Central South University,Changsha,Hunan,410000;Dalian Medical University,Dalian,Liaoning,116000) |
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| Keywords: | GKT137831 atorvastatin reactive oxygen species glucose uptake |
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