| 12味止血中药对脂多糖诱导小鼠巨噬细胞产生一氧化氮的抑制作用 |
| |
| 引用本文： | 廖晖,Linda K Banbury,David N Leach. 12味止血中药对脂多糖诱导小鼠巨噬细胞产生一氧化氮的抑制作用[J]. 中国药房, 2007, 0(9) |
| |
| 作者姓名： | 廖晖 Linda K Banbury David N Leach |
| |
| 作者单位： | 山西省人民医院药剂科 太原市030012(廖晖)，澳大利亚南十字星大学植物化学及药理学研究中心 新南威尔士州2480(Linda K Banbury，David N Leach) |
| |
| 摘 要： | 目的:研究12味止血中药对脂多糖(LPS)诱导小鼠巨噬细胞产生一氧化氮(NO)的抑制作用,并探讨其止血机制。方法:以1μg.mL-1LPS刺激小鼠巨噬细胞RAW264.7,22h后以Griess法测定NO的终产物亚硝酸盐的含量,观察LPS对NO生成的影响。结果:12味止血药均可抑制LPS诱导小鼠巨噬细胞RAW264.7中NO的生成。三七、地榆、仙鹤草、槐花、艾叶、蒲黄、侧柏叶及白茅根的抑制作用显著(P<0.05)。结论:本实验为12味止血中药对脂多糖诱导小鼠巨噬细胞产生NO的抑制作用提供了一定的理论依据。
|
| 关 键 词： | 止血中药 脂多糖 一氧化氮 巨噬细胞RAW264.7 |
Inhibitory Effects of 12 Hemostatic Chinese Herbs on Nitric Oxide Production in LPS-Activated RAW 264.7 Macrophages |
| |
| LIAO Hui. Inhibitory Effects of 12 Hemostatic Chinese Herbs on Nitric Oxide Production in LPS-Activated RAW 264.7 Macrophages[J]. China Pharmacy, 2007, 0(9) |
| |
| Authors: | LIAO Hui |
| |
| Abstract: | OBJECTIVE:To investigate the inhibitory effects of 12 hemostatic Chinese herbs on Nitric Oxide production in lipopolysaccharide(LPS)activated RAW 264.7 Macrophages.METHODS:Nitric Oxide production was determined with Griess assay after 22 hrs stimulation of 1 μ g· mL-1 LPS on RAW 264.7.Nitrite concentrations were determined by comparison with a sodium nitrite standard curve.RESULTS:The markedly increased production of nitrite by stimulation of RAW 264.7 macrophages with LPS was inhibited significantly(P<0.05)by:Panax Notoginseng,Sanguisorba Officinalis,Agrimonia Pilosa,Sophora Japonica,Artemisia Argyi,Typha Angustifolia,Platycladus Orientalis and Imperata Cylindrical.CONCLUSION:The hemostatic action of 12 herbs may be related to their inhibitory effect on Nitric Oxide production. |
| |
| Keywords: | Hemostatic Chinese herbs LPS Nitric Oxide RAW 264.7 macrophages |
| 本文献已被 CNKI 等数据库收录！ |
