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| 半夏种质资源的随机扩增多态性DNA技术分析 | |
| 引用本文： | 杨俊宝,朱秀志,罗成科,李俊,魏会廷,彭正松.半夏种质资源的随机扩增多态性DNA技术分析[J].中国中医药信息杂志,2007,14(1):42-44,91. |
| 作者姓名： | 杨俊宝 朱秀志 罗成科 李俊 魏会廷 彭正松 |
| 作者单位： | 1. 西华师范大学生命科学学院,四川,南充,637002;川北医学院医学生物学教研室,四川,南充,637007 2. 西华师范大学生命科学学院,四川,南充,637002;赣南医学院基础医学部,江西,赣州,341000 3. 宁夏大学新技术应用研究开发中心,宁夏,银川,750002 4. 西华师范大学生命科学学院,四川,南充,637002 |
| 基金项目： | 四川省重点学科建设项目;四川省杰出青年科学基金 |
| 摘 要： | 目的分析半夏种质资源在分子水平上的遗传多样性。方法应用随机扩增多态性DNA技术(RAPD)对21份半夏种质资源进行检测。结果29个引物共得到162条扩增DNA片段,其中123条(75.9%)的片段具多态性,揭示了半夏居群间较丰富的遗传多样性。利用UPGMA法进行聚类分析表明,所有材料可划分为3类,根据RAPD遗传相似系数划分的类群同地理分布有一定关系。结论半夏种质资源在分子水平上确实存在较大遗传差异。RAPD标记可作为构建半夏DNA指纹图谱的有效工具。 |
| 关 键 词： | 半夏 随机扩增多态性DNA技术 遗传多样性 DNA指纹图谱 |
| 文章编号： | 1005-5304（2007）01-0042-03 |
| 收稿时间： | 2006-05-15 |
| 修稿时间： | 2006-05-15 |
RAPD Analysis on the Germplasm Resources of Pinellia Ternata |
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| YANG Jun-bao, ZHU Xiu-zhi, LUOCheng-ke, et al.RAPD Analysis on the Germplasm Resources of Pinellia Ternata[J].Chinese Journal of Information on Traditional Chinese Medicine,2007,14(1):42-44,91. | |
| Authors: | YANG Jun-bao ZHU Xiu-zhi LUOCheng-ke |
| Abstract: | Objective To discuss the genetic diversity of the germplasm resources of Pinellia ternata on molecular level. Methods The genetic diversity of P. ternata 21 different populations were tested by RAPD markers. Results A total of 162 products were amplified by 29 10-mer arbitrary primers, 123 (75.9%) products were found to be polymorphic. The results revealed the abundant genetic diversity in P. ternata 21 different populations. The cluster analysis using UPGMA method showed that 21 populations were classified as 3 groups, and the genetic diversity in P. ternata based on RAPD GS was correlated with geographic distribution. Conclusion There actually existed much genetic diversity on molecular level among the germplasm resources of P. ternata. RAPD markers could be effective tools to construct DNA fingerprintings of P. ternata. |
| Keywords: | Pinellia ternata RAPD genetic diversity DNA fingerprintings |
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