新生大鼠视神经少突胶质细胞原代培养及鉴定

目的：探讨视神经少突胶质细胞的分离方法和体外培养条件，为研究视神经损伤修复及少突胶质细胞相关课题研究奠定基础。方法：新生大鼠视神经取材，以DMEM／F12为基础的化学限定性培养基进行组织块培养，并用少突胶质细胞特异性标记物GC鉴定细胞。结果：培养第3天，视神经周围出现少突胶质细胞生长，呈圆形或梭形，10d左右基本铺满盖玻片。免疫细胞化学检测该细胞为阳性细胞，纯度较高。结论：采用视神经组织并用化学限定性培养基能成功获取体外培养的少突胶质细胞、较传统方法有获得细胞量大、纯度高、操作简便易行等特点。

The culture and identification of oligodendrocyte of rats optic nerve in vitro

Objective To investgate the isolation method and growth condition of oligodendrocyte of optic nerve,and establish an approach to the research of physiologic function and repair of injury of optic nerve and related diseases.Methods Optic nerve of newborn rat was isolated and DMEM/F12 chemically defined culture medium was used to culture oligodendrocyte.The morphologic and growth alterations of the cells were observed,then the cells were identified by specific marker(GC)through immunbocytochemical examination.Results The cells migrated from optic nerve at the third day in round or fusiform.The cover slips were overgrowed about 10 days later.Immunocytochemical examination showed that the cells obtained by this method were oligodendrocytes.Conclusion The method we used can gain more and purer cells than conventional method.The operation is convenient and easy.