Dual effect of flurbiprofen on cell proliferation and agonist-induced Ca(2+) movement in human osteosarcoma cells |
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Authors: | Tseng Li-Ling Cheng He-Hsiung Huang Chun-Jen Liu Shiuh-Inn Kuo Chun-Chi Chen Wei-Chuan Huang Jong-Khing Hsu Shu-Shong Chang Hong-Tai Kao Cheng-Hsing Ho Chin-Man Jan Chung-Ren |
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Institution: | Department of Dentistry, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan 813. |
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Abstract: | In human MG63 osteosarcoma cells, the effect of flurbiprofen on intracellular Ca(2+) concentrations (Ca(2+)](i)) and proliferation was explored. The proliferation was enhanced by 20-120 microM flurbiprofen, and was decreased by 140-200 microM flurbiprofen. The effect of flurbiprofen on the increases in cytosolic free Ca(2+) levels (Ca(2+)](i)) induced by ATP, bradykinin, histamine and thapsigargin (an inhibitor of the endoplasmic reticulum Ca(2+) ATPase), was examined. In cell preincubated with 20 or 80 microM flurbiprofen, the Ca(2+)](i) increases induced by all agonists were attenuated. In the presence of 20 microM flurbiprofen, the decreased Ca(2+)](i) responses with the agonists were attributed to a defective Ca(2+) influx because this decrease was unobserved in agonists-induced Ca(2+)](i) increases in the absence of extracellular Ca(2+). In the presence of 80 microM flurbiprofen, both the Ca(2+) influx component and the Ca(2+) releasing (from organelles) component were defective. These results suggest that flurbiprofen could alter proliferation and inhibit Ca(2+)](i) increases. |
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