阿托伐他汀对心肌细胞缺氧／复氧损伤的保护作用

目的探讨阿托伐他汀保护心肌细胞缺氧／复氧损伤的机制，为临床防治缺血再灌注损伤寻找新思路。方法分离SD乳鼠（出生1～3d）心肌细胞进行原代培养，建立H/R模型，取培养第3d的心肌细胞随机分成3组：正常对照组（CON组）、H，R组、H／R＋ATV组。应用MTS法检测心肌细胞的存活率；化学发光免疫分析法检测心肌细胞特异性损伤指标肌钙蛋白I（cTnI）的含量；采用SYBRGREEN荧光定量PCR检测各组HIF—1a、VEGFmRNA表达水平。结果与CON组比较，H／R组心肌细胞存活率明显降低（P〈0．05），血清肌钙蛋白I明显升高（P〈O．05），HIF—1a和VEGFmRNA表达水平显著升高（P〈0．05）；用阿托伐他汀预处理后心肌细胞存活率明显升高（P〈0．05），血清肌钙蛋白I明显降低（P〈0．05），HIF-1d和VEGFmRNA表达水平进一步升高（P〈0．05）。结论阿托伐他汀可能通过上调HIF-1a及VEGF的表达水平进而保护缺氧／复氧损伤的心肌细胞。

Protection of atorvastatin in cardiomyocytes induced by hypoxia/reoxygenation injury

Objective To discuss the mechanisms of protection of cardiomyocytes during hypoxia/reoxygenation injury, provide new ideas for the prevention and treatment in myocardial ischemia/reperfusion injury clinically. Methods Cardiomy- ocytes from SD rats （1-3d） were separated for primary culture and established hypoxiaJreoxygenation models; the cardiomyocytes cultured on the third day were divided into three groups randomly： CON group, H/R group and H/R＋ATV group. Used the MTS assay to detect the cardiomyocytes survival rate; the level of serum troponin I （cTnI） was detected by chemil munoassay analyzer; used SYBR GREEN fluorescent quantitative PCR to detect the expression of HIF-lct,VEGF mRNA. Re- sults Compared with CON group, cardiomyocytes survival rate of H/R group beating rate and cell livability decreased obviously （P〈0.05）, serum troponin I was higher clearly （P〈0.05）, the expression of HIF-lo~,VEGF mRNA were increased significantly （P〈0.05）; compared with H/R group, cardiomyocytes survival rate of H/R＋ATV group increased obviously （P〈0.05）, serum troponin I was lower clearly（P〈0.05）, the expression of HIF-la,VEGF mRNA were significantly higher（P〈0.05）. Conclusion Atorvastatin may raise the expression of HIF-la and VEGF to protect myocardial cells induced by hypoxia/reoxygenation injury.