杭白菊总黄酮抗炎作用及其机制初探

目的：观察杭白菊总黄酮抗炎作用并对其机制进行初步研究。方法：采用二甲苯致小鼠耳肿胀、醋酸致小鼠腹腔毛细血管通透性增加、角叉菜胶致小鼠足跖肿胀及大鼠气囊炎炎症模型,观察杭白菊总黄酮对不同炎症模型的抗炎作用,并测定蛋白质、白细胞数（WBC）、前列腺素E2（PGE2）、丙二醛（MDA）含量及超氧化物歧化酶（SOD）活性。结果：杭白菊总黄酮能显著降低小鼠毛细血管通透性,抑制小鼠二甲苯致耳肿胀,减轻小鼠足跖肿胀,降低炎症组织PGE2含量,抑制角叉菜胶致大鼠气囊炎模型中渗出液中总蛋白质含量和白细胞数,降低血浆MDA含量,增强血浆SOD活性。结论：杭白菊总黄酮具有明显的抗炎作用,其抗炎作用可能与其降低血管通透性、抑制PGE2等炎症介质生成及增强清除氧自由基、抗脂质过氧化能力有关。

Anti-inflammatory effect and mechanism study of total flavonoids in Chrysanthemum morif olium Ramat

AIM: To study the anti-inflammatory effect of total flavonoids in Chrysanthemum morif olium Ramat and the mechanism. METHODS: The auricle edema was induced by dimethylbenzene, the abdominal cavity capillary permeability was increased by Hac, the edema in feet was induced by carrageenan in mice, and the air-pouch acute inflammation induced by carra-geenan in rats were applied in anti-inflammatory experi-ments. The anti-inflammatory effect of total flavonoids in different models were observed, then the content of the protein, the amount of white blood cell (WBC), prostaglandin E_2(PGE_2) and malonaldehyde (MDA) and the activity of superoxide dismutase (SOD) were determined. RESULTS: Total flavonoids could inhibit the capillary permeability and the engorgement of in-flammatory texture, decrease the total protein content in efflusion and the amount of WBC, reduce PGE_2 and MDA contents and increase SOD activity. CONCLU-SION: The mechanisms of anti-inflammatory action of total flavonoids are related to the depression of vasoper-meability, reduction of inflammatory mediators and augmentation of oxygen free radical scavenging capacity and anti lipid peroxidation.