老化豚鼠耳蜗毛细胞和螺旋神经节细胞定量研究

本文对3～3.5年老化豚鼠进行毛细胞和螺旋神经节细胞定量研究,并与1.5月龄青年豚鼠比较。结果表明:老化豚鼠外毛细胞损失率明显高于青年组（P<0.01）,而内毛细胞损失率无明显差异。外毛细胞损失率OHC3>OHC2>OHC1。顶回和底回螺旋神经节细胞分别减少15％和19.81％。耳蜗中轴PAS染色,螺旋神经节细胞内大量脂褐素Lp增加,柯替氏器不同程度退变,血管纹及螺旋韧带萎缩程度轻,耳蜗神经纤维减少,但胶质细胞增加。讨论了老化豚鼠形态变化对听功能的影响。

A quantitative study on the cochlear hair cell and spiral ganglion cell in senile guinea pigs

A quantitative study on the cochlear hair cell and spiral ganglion cell was carried out in guinea pigs of two age groups (36-42, 1. 5 months). The surface specimen technique was used for the study of cochlear hair cell loss in both groups. Damage rate of outer hair cells was higher in the older group than in the younger (P<0. 01), while the damage rates of inner hair cells showed no significant difference between these two groups, Outer hair cell loss was greatest in the apex of the cochlea, followed by the basal coil. Of these cells, the third row was most seriously affected: OHC3>OHC2>OHC1. The number of spiral ganglion cells was reduced by 15% in the apex and 19. 81% in the basal coil. Mid-modiolus sections of cochlea stained by PAS showed a large amount of lipofusion (Lp) in ganglion cells. The organ of Corti showed various degenerative changes, while the atrophic changes of stria vascularis and spiral ligament were less prominent, especially in the apex of cochlea. The morphological changes of cochlear nerve in the older animals showed declination of fibers and increase of glial cells.The association of the changes in morphology with the hearing loss in the senile guinea pigs are discussed.