| 大茴香酸-硫酸荧光体系测定黄芪甲苷 |
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| 引用本文： | 刘养清,杜鸣,徐秉玖.大茴香酸-硫酸荧光体系测定黄芪甲苷[J].药学学报,2000,35(7):544-546. |
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| 作者姓名： | 刘养清 杜鸣 徐秉玖 |
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| 作者单位： | 1. 山西师范大学化学系,山西,临汾,041004
2. 北京医科大学药学院分析化学与药物分析研究室,北京,100083 |
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| 摘 要： | 黄芪甲苷 (ａｓｔｒａｇａｌｏｓｉｄｅ)是中药膜荚黄芪Ａｓｔｒａｇａｌｕｓｍｅｍｂｒａｎａｃｅｕｓ (Ｆｉｓｃｈ .)Ｂｇｅ .和蒙古黄芪Ａｓｔｒａｇａｌｕｓｍｅｍｂｒａｎａｃｅｕｓ (Ｆｉｓｃｈ .)Ｂｇｅ .ｖａｒ .ｍｏｎｇｈｏｌｉｃｕｓ (Ｂｇｅ .)Ｈｓｉａｏ的主要活性成分 ,有抗炎、降压、镇痛、镇静、升高血浆中ｃＡＭＰ水平、促进小鼠再生肝ＤＮＡ的含量1,2 ] 以及促进免疫功能等生理活性。黄芪甲苷的测定方法主要有 :紫外分光光度法3 ,4 ] 、薄层扫描法5,6] 和ＨＰＬＣ法7,8] 。光度法常用香草醛在浓硫酸作用下与甲苷显色…
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| 关 键 词： | 黄芪甲苷 大茴香酸 黄芪 中药复方补阳还五汤 荧光分光光度法 |
| 收稿时间： | 1999-08-03 |
| 修稿时间： | : |
DETERMINATION OF ASTRAGALOSIDE IN ANISIC ACID-SULPHURIC ACID SYSTEM BY SPECTROPHOTOFLUORIMETRY |
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| LIU Yang-Qing,DU Ming,XU Bing-Jiu.DETERMINATION OF ASTRAGALOSIDE IN ANISIC ACID-SULPHURIC ACID SYSTEM BY SPECTROPHOTOFLUORIMETRY[J].Acta Pharmaceutica Sinica,2000,35(7):544-546. |
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| Authors: | LIU Yang-Qing DU Ming XU Bing-Jiu |
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| Abstract: | AIM To develop a new determination method of astrogaloside in anisic acid-sulfuric acid system by spectrophotofluorimetry. METHODS Using 2% anisic acid anhydrous alcoholic solution as colour developing reagent in the presence of sulfuric acid to determine astragaloside in pig serum. The reaction was allowed to procced on a 60℃ water-bath for 20 min. RESULTS The reaction product of astrogaloside and anisic acid has excitation and emission maxima at 320 and 387 nm, respectively. The linear range of determination is from 0.2 to 40 μg*mL-1. The detection limit is 0.02 μg*mL-1. The recoveries of samples are between 98.65% and 102.4%. CONCLUSION The proposed method has advantages of high sensitivity, low detection limit and no interference. It has been used to determine several kinds of samples successfully. |
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| Keywords: | astragaloside anisic acid Astragalus membranaceus Bge compound medicine Bu Yang Huan Wu Tang spectrophotofluorimetry |
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