尾加压素Ⅱ及其受体在大鼠肾成纤维细胞中的表达及银杏叶的干预作用

目的：探讨血管紧张素Ⅱ（AngⅡ）对大鼠肾间质成纤维细胞尾加压素Ⅱ（UⅡ）及其受体（GPR14）表达的影响和银杏叶(GBE）的干预作用。方法：体外培养大鼠肾成纤维细胞，以未处理细胞作为正常对照组，以AngⅡ作为刺激因素，分别加入终浓度为0、25、50、100和200 g?L^-1的GBE，RT-PCR检测各组细胞UⅡ及GPR14 mRNA表达，免疫印迹法检测各组细胞UⅡ及GPR14蛋白含量，ELISA法检测培养上清中Ⅰ型胶原（ColⅠ）含量。结果： AngⅡ组UⅡ、GPR14表达量及培养上清中ColⅠ含量明显高于正常对照组（P<0.01），与AngⅡ刺激组比较，AngⅡ+GBE组UⅡ及GPR14的mRNA表达量下降（P<0.01），蛋白含量减少（P<0.01或P<0.05），培养上清中ColⅠ含量明显降低（P<0.01或P<0.05）。结论：GBE可抑制AngⅡ诱导的肾间质成纤维细胞UⅡ及受体mRNA表达，降低其蛋白含量，减少细胞外基质ColⅠ分泌。

Expression of urotensin Ⅱ and its receptor GPR14 in renal interstitial fibroblasts and intervention of ginkgo bioba extract

Objective To explore the effects of angiotensinⅡ products on urotensinⅡ(UⅡ) and its receptor GPR14 expression in cultured renal interstitial fibroblasts and the intervention of ginkgo bioba extract(GBE). Methods The rat renal fibroblasts were cultivated in vitro,untreated cells were acted as normal control group,AngⅡ as the stimulating factor,the cells were treated with GBE with concentrations of 0,25,50,100 and 200 g·L-1,the mRNA and protein levels of UⅡ and GPR14 were measured by RT-PCR and Western blotting,the content of collagenⅠ in supernatant was detected by ELISA method. Results Compared with AngⅡ group,the mRNA and protein levels of UⅡ and GPR14 were significantly down-regulated (P<0.01 or P<0.05),the contents of ColⅠ in supernatant were markedly decreasedin AngⅡ+GBE group(P<0.01 or P<0.05). Conclusion GBE can down regulate the UⅡ and GPR14 mRNA expression,decrease the protein levels of UⅡ and GPR14,and reduce the ColⅠ secretion in cultured fibroblasts.